Microbiology lab final NDSU Questions and
Correct Answers the Latest Update
Primary stain
✓ crystal violet- purple dye
What is the purpose of IMViC testing?
✓ To distinguish between fecal and non fecal coliforms?
Selective media
✓ grow specific microorganisms and withhold others
What are non-iodizing radiation
✓ visible light and ultraviolet light
ultraviolet radiation damages bacteria by introducing....
✓ thymine dimers
Growth media
✓ essential nutrients needed to grow microbes in lab
From exercise 11, where we inoculated glucose broth tubes (containing bromcresol purple as a
pH indicator) with bacteria. Describe the results for typical thermophile and mesophile
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✓ Mesophile will be purple at 55c and Thermophile will be yellow at 55c
Nutrient agar
✓ nutrient broth with added sugar to make it solid
Tempered agar
✓ melted and cooled agar
The planting of Serratia Marcescenes onto agar containing a low level of streptomycin (NAS)
resulted in organisms that developed a resistance to the antibiotic by...
✓ Random mutations
What does it mean when an organism cant grow in the presence of oxygen
✓ it is obligate anaerobe
_____ bacteria have high lipid content in their cell walls and retain the carbol fushin stain
even after washing with acidifed- alcohol
✓ Acid Fast
Acid fast stain is important because it diagnosis's ...
✓ Tuberculosis
The gram stain differentiates between bacteria based on the composition of their:
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✓ Cell wall
Acid fast bacteria stain what color
✓ Red
What does alpha hemolysis look like
✓ Green blob
What does beta hemolysis look like
✓ In the picture it appears yellows streaks
What does gamma hemolysis look like
✓ White/ clear streaks
Looking at a mannitol salt agar plate, which side would show the growth of S. aureus?
And how do you know??
✓ The yellow side because of the selective media. Fermentation = yellow
Why are multi drug resistant bacteria an important healthcare concern? What can we do to
combat the problem?
✓ B/C bacteria is becoming resistant fast. Helps if you listen to your doctor. Take as
prescribed. Dont over use. Do not stop medication early
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