Medical Interventions EOC Exam Questions And
100% Correct Answers
The new vaccine was developed using killed microbes that cause influenza. The killed
cells have antigens on their surfaces which are recognizable and can elicit an immune
response. However, they cannot cause disease. To which class of vaccines does the
new vaccine belong? - Answer Inactivated whole agent
Why must DNA be heated to 94 degrees C in the first step of the polymerase chain
reaction cycle? - Answer to break hydrogen bonds between DNA strands
Beta-lactam antibiotics, such as penicillin, inhibit the action of bacterial enzymes that
are responsi- ble for. - Answer degrading the peptidoglycan cell wall
You work in quality control at a biomanufactoring company and you have to hire
someone to check on the purity of a manufactured protein. What would be the most
important skill that someone you hire could possess? - Answer Experience running and
analyzing results of protein ekectrophoresis
Sensorineural hearing loss is caused by. - Answer damage to the auditory nerve
Hydrophobic interactive chromatography is a technique of purifying a protein based on.
- Answer its hydrophobic properties
Cochlear implants could possibly help a person who has a problem with their. - Answer
inner ear
The parts of the ear that converts stimulus from the outside environment into the nerve
impulses for transmission to the brain is the. - Answer cochlea
,DNA ligase is ________Answer A linking enzyme that links pieces of DNA together
Recombinant DNA can be inserted into a bacteria using a bacteriophage through the
process of. ________Answer transduction
Epidemiology is the ________Answer study of bacterial infections and how they are
transferred in a population
A bacterial culture is several days old. Most of the bacterial cells have lysed and leaked
DNA into the nutrient agar. A few living bacteria cells in the culture have become
genetically transformed by taking the DNA into the cytoplasm and are now resistant to
amplicillin. The above describes which of the following processes? - Answer
Transformation
ELISA test During the test, the secondary antibody was conjugated with an enzyme.
What happens when that enzyme was attached with a substrate? Answer the substrate
causes the enzyme to oxidize resulting in color change
A tumor suppressor gene is a gene that. Answer produces proteins that inhibit cell
division
DNA micro-arrays measure Answer the amount of cDNA for every gene that is
expressed
Which of the following antibiotics blocks the attachment of tRNA to the ribosome
preventing protein synthesis - Answer tetracylines
What bio-medical professionals are involved in the stages of producing and
manufacturing a protein product - Answer genetic engineers isolate and insert gene
microbiologists culture bacteria
lab techs generate/purify protein
pharmacologists design/test medications
, What role does insulin play in diabetes? - Answer INSULIN allows cells to take up sugar,
getting sugar out of the blood
Type 1 diabetics must receive insulin shots to live—otherwise their cells will die (require
glucose to make energy
How has the diagnosis and treatment for diabetes changed within the past 200 years? -
Answer Long ago-diagnosed by tasting pee for sweetness
1700's-1800's-treated with dietary restrictions & deadly chemicals
1920s on-insulin used to treat patients
How are bacterial plasmids used to produce proteins like insulin? - Answer This is done
through genetic engineering
Plasmids are isolated from bacteria
Plasmid is cut with restriction enzyme
The gene for insulin (or other protein) is inserted into the plasmid
Plasmid is closed (it now contains recombinant DNA—DNA from multiple sources)
Bacteria are made to take up plasmid and pump out protein
What is bacterial transformation? - Answer Transformation is the process by which cells
are made to take up a genetically engineered plasmid
then they will express the protein for which the inserted gene(s) code Transformed
bacteria are cultured on antibiotic such as ampicillin to ensure purity of culture
How would you quantify the success of a transformation experiment? - Answer You
would need to determine the transformation efficiency, which is how many
transformants there were per microgram of DNA added
How is amino acid structure related to the overall shape of a protein? - Answer: The
proteins consist of amino acids that are hydrophilic (water-loving) and others that are
hydrophobic (water-fearing)
100% Correct Answers
The new vaccine was developed using killed microbes that cause influenza. The killed
cells have antigens on their surfaces which are recognizable and can elicit an immune
response. However, they cannot cause disease. To which class of vaccines does the
new vaccine belong? - Answer Inactivated whole agent
Why must DNA be heated to 94 degrees C in the first step of the polymerase chain
reaction cycle? - Answer to break hydrogen bonds between DNA strands
Beta-lactam antibiotics, such as penicillin, inhibit the action of bacterial enzymes that
are responsi- ble for. - Answer degrading the peptidoglycan cell wall
You work in quality control at a biomanufactoring company and you have to hire
someone to check on the purity of a manufactured protein. What would be the most
important skill that someone you hire could possess? - Answer Experience running and
analyzing results of protein ekectrophoresis
Sensorineural hearing loss is caused by. - Answer damage to the auditory nerve
Hydrophobic interactive chromatography is a technique of purifying a protein based on.
- Answer its hydrophobic properties
Cochlear implants could possibly help a person who has a problem with their. - Answer
inner ear
The parts of the ear that converts stimulus from the outside environment into the nerve
impulses for transmission to the brain is the. - Answer cochlea
,DNA ligase is ________Answer A linking enzyme that links pieces of DNA together
Recombinant DNA can be inserted into a bacteria using a bacteriophage through the
process of. ________Answer transduction
Epidemiology is the ________Answer study of bacterial infections and how they are
transferred in a population
A bacterial culture is several days old. Most of the bacterial cells have lysed and leaked
DNA into the nutrient agar. A few living bacteria cells in the culture have become
genetically transformed by taking the DNA into the cytoplasm and are now resistant to
amplicillin. The above describes which of the following processes? - Answer
Transformation
ELISA test During the test, the secondary antibody was conjugated with an enzyme.
What happens when that enzyme was attached with a substrate? Answer the substrate
causes the enzyme to oxidize resulting in color change
A tumor suppressor gene is a gene that. Answer produces proteins that inhibit cell
division
DNA micro-arrays measure Answer the amount of cDNA for every gene that is
expressed
Which of the following antibiotics blocks the attachment of tRNA to the ribosome
preventing protein synthesis - Answer tetracylines
What bio-medical professionals are involved in the stages of producing and
manufacturing a protein product - Answer genetic engineers isolate and insert gene
microbiologists culture bacteria
lab techs generate/purify protein
pharmacologists design/test medications
, What role does insulin play in diabetes? - Answer INSULIN allows cells to take up sugar,
getting sugar out of the blood
Type 1 diabetics must receive insulin shots to live—otherwise their cells will die (require
glucose to make energy
How has the diagnosis and treatment for diabetes changed within the past 200 years? -
Answer Long ago-diagnosed by tasting pee for sweetness
1700's-1800's-treated with dietary restrictions & deadly chemicals
1920s on-insulin used to treat patients
How are bacterial plasmids used to produce proteins like insulin? - Answer This is done
through genetic engineering
Plasmids are isolated from bacteria
Plasmid is cut with restriction enzyme
The gene for insulin (or other protein) is inserted into the plasmid
Plasmid is closed (it now contains recombinant DNA—DNA from multiple sources)
Bacteria are made to take up plasmid and pump out protein
What is bacterial transformation? - Answer Transformation is the process by which cells
are made to take up a genetically engineered plasmid
then they will express the protein for which the inserted gene(s) code Transformed
bacteria are cultured on antibiotic such as ampicillin to ensure purity of culture
How would you quantify the success of a transformation experiment? - Answer You
would need to determine the transformation efficiency, which is how many
transformants there were per microgram of DNA added
How is amino acid structure related to the overall shape of a protein? - Answer: The
proteins consist of amino acids that are hydrophilic (water-loving) and others that are
hydrophobic (water-fearing)
