(MB) ASCP Practice(2024-2025) Exam Questions with
Answers
This translocation creates a fusion protein between the Abl1
gene on one chromosome and the BCR gene on the other
chromosome, resulting in chronic myelogenous leukemia
(CML): - <<<<Answers>>>t(9;22)
What stain binds to minor groove of DNA Duplex? -
<<<<Answers>>>SYBR1
Bone marrow transplantation is a great method used to treat
several malignant and benign cancers, particularly leukemias.
Before transplantation (or engraftment analysis), several
polymorphic loci are screened in both the recipient and donor
cells. The main goal of this screening is to:
What is the advantage of Next Generation Sequencing (NGS)
over traditional Sanger Sequencing?
-Ability to sequence multiple mutation variants of a single
gene at a single time
-Preparation of a library
-Easy setup
-Use of a barcode reader - <<<<Answers>>>Ability to
sequence multiple mutation variants of a single gene at a
single time
What links codons and anti-codons together during DNA
translation?
,(MB) ASCP Practice(2024-2025) Exam Questions with
Answers
DNA ligase
Single-stranded binding protein
Complementary base pairing
GTP - <<<<Answers>>>Complementary base pairing
Both parents are carriers of an autosomal recessive disorder.
What percent chance do these parents combined have to pass
down this affected gene to their child?
0%
25%
50%
75%
100% - <<<<Answers>>>75%
A cerebrospinal fluid (CSF) sample for hematologic
evaluation should be tested within how many hours of
collection?
1 hour
2 hours
3 hours
4 hours - <<<<Answers>>>1 hour
Blood drawn into what type of tube is compatible for Genetic
testing?
,(MB) ASCP Practice(2024-2025) Exam Questions with
Answers
K2EDTA tube
Sodium citrate tube
Sodium Heparin tube
Boric acid, sodium formate tube -
<<<<Answers>>>K2EDTA tube
-find at least one or more informative loci between both the
donor and recipient
-find exactly ten informative loci between both the donor and
recipient
-find all the non-informative loci between both the donor and
recipient
-find the blood type/group of both the donor and recipient -
<<<<Answers>>>find at least one or more informative loci
between both the donor and recipient
A DNA specimen was sent to your Molecular Diagnostics
Laboratory for DNA sequencing, in order to determine
whether patient A has a particular mutation in gene X. The
gene sequence is known. However, the gene mutation itself is
not known. Therefore, DNA sequencing cannot be performed
on this DNA specimen. (True or False)
True
False - <<<<Answers>>>True
After an amplification procedure followed by gel
electrophoresis, you took a photo of your gel. You notice
, (MB) ASCP Practice(2024-2025) Exam Questions with
Answers
consistent bands in all your gel lanes. However, you also see
what appears to be a faint but noticeable band in your reagent
blank lane. What is your best explanation for the reagent
blank band?
-Taq polymerase concentration was too low in this PCR
reaction
-There was obvious contamination in this PCR reaction
-The primers concentration was too high in this PCR reaction
-The dNTPs concentration was too high in this PCR reaction -
<<<<Answers>>>There was obvious contamination in this
PCR reaction
In mantle cell lymphoma (MCL), this is the fusion gene
created:
EWS-FLI1
CCND1-IGH
BCR-IGH
c-myc-EWS - <<<<Answers>>>CCND1-IGH
This enzyme is known for "unzipping" genes!
Alkaline Phosphatase
RNA polymerase
Apyrase
Helicase - <<<<Answers>>>Helicase
Answers
This translocation creates a fusion protein between the Abl1
gene on one chromosome and the BCR gene on the other
chromosome, resulting in chronic myelogenous leukemia
(CML): - <<<<Answers>>>t(9;22)
What stain binds to minor groove of DNA Duplex? -
<<<<Answers>>>SYBR1
Bone marrow transplantation is a great method used to treat
several malignant and benign cancers, particularly leukemias.
Before transplantation (or engraftment analysis), several
polymorphic loci are screened in both the recipient and donor
cells. The main goal of this screening is to:
What is the advantage of Next Generation Sequencing (NGS)
over traditional Sanger Sequencing?
-Ability to sequence multiple mutation variants of a single
gene at a single time
-Preparation of a library
-Easy setup
-Use of a barcode reader - <<<<Answers>>>Ability to
sequence multiple mutation variants of a single gene at a
single time
What links codons and anti-codons together during DNA
translation?
,(MB) ASCP Practice(2024-2025) Exam Questions with
Answers
DNA ligase
Single-stranded binding protein
Complementary base pairing
GTP - <<<<Answers>>>Complementary base pairing
Both parents are carriers of an autosomal recessive disorder.
What percent chance do these parents combined have to pass
down this affected gene to their child?
0%
25%
50%
75%
100% - <<<<Answers>>>75%
A cerebrospinal fluid (CSF) sample for hematologic
evaluation should be tested within how many hours of
collection?
1 hour
2 hours
3 hours
4 hours - <<<<Answers>>>1 hour
Blood drawn into what type of tube is compatible for Genetic
testing?
,(MB) ASCP Practice(2024-2025) Exam Questions with
Answers
K2EDTA tube
Sodium citrate tube
Sodium Heparin tube
Boric acid, sodium formate tube -
<<<<Answers>>>K2EDTA tube
-find at least one or more informative loci between both the
donor and recipient
-find exactly ten informative loci between both the donor and
recipient
-find all the non-informative loci between both the donor and
recipient
-find the blood type/group of both the donor and recipient -
<<<<Answers>>>find at least one or more informative loci
between both the donor and recipient
A DNA specimen was sent to your Molecular Diagnostics
Laboratory for DNA sequencing, in order to determine
whether patient A has a particular mutation in gene X. The
gene sequence is known. However, the gene mutation itself is
not known. Therefore, DNA sequencing cannot be performed
on this DNA specimen. (True or False)
True
False - <<<<Answers>>>True
After an amplification procedure followed by gel
electrophoresis, you took a photo of your gel. You notice
, (MB) ASCP Practice(2024-2025) Exam Questions with
Answers
consistent bands in all your gel lanes. However, you also see
what appears to be a faint but noticeable band in your reagent
blank lane. What is your best explanation for the reagent
blank band?
-Taq polymerase concentration was too low in this PCR
reaction
-There was obvious contamination in this PCR reaction
-The primers concentration was too high in this PCR reaction
-The dNTPs concentration was too high in this PCR reaction -
<<<<Answers>>>There was obvious contamination in this
PCR reaction
In mantle cell lymphoma (MCL), this is the fusion gene
created:
EWS-FLI1
CCND1-IGH
BCR-IGH
c-myc-EWS - <<<<Answers>>>CCND1-IGH
This enzyme is known for "unzipping" genes!
Alkaline Phosphatase
RNA polymerase
Apyrase
Helicase - <<<<Answers>>>Helicase
