BIO 353 Study Guide Exam 1 Questions And Answers Graded A+.

BIO 353 Study Guide Exam 1 Questions And Answers Graded A+. The cell - correct answer. The basic unit of structure and function in living things What are the major Organelles/ structures of a basic eukaryotic cell? - correct answer. nucleus nucleolus ribosomes smooth ER rough ER mitochondria What are the 3 major disciplines involved with studying biology? - correct answer. bioimaging biochemistry mol. biology/genetics What is light microscopy? - correct answer. visible light passes through a series of lenses to produce a magnified image What is the resolution limit of the light microscope? - correct answer. .2 nm Define resolution - correct answer. Describes the degree of clarity, detail, and sharpness of an image Define magnification - correct answer. the ratio of an object's image size to its real size what is one of the major advantages of the basic light microscope? - correct answer. ability to image LIVE cells State of cells when using amplitude contrast - correct answer. fixed/dead Describe amplitude contrast - correct answer. a form of contrast generated by the absorption of selective wave lengths from the visible light spectrum Describe phase contrast microscopy - correct answer. dependent on light refraction contrast formed b/c different parts have different densities State of cells when using phase contrast? - correct answer. living epifluorescence microscopy - correct answer. detection of specific molecules/ions/organelles that are MADE fluorescent by coupling them to a flourochrome marker indirect immunofluorescence - correct answer. i. cells/tissues fixed ii. exposed to primary antibody, antibody binds to specific protein (ie antigen) iii. exposed to secondary antibody that is coupled to a flourochrome marker What is GFP and how is it coupled to the proteins in different cellular structures (direct method)? - correct answer. Green Fluorescent Protein binds the flourescent sntibody to the corresponding antigen on the target protein Whats the advantage of using GFP? - correct answer. cells are ALIVE What are vital fluorescent dyes (direct method)? - correct answer. harmless dyes used to stain living tissue for observation confocal microscopy - correct answer. a light microscope that uses fluorescent stains and laser to make two- and three-dimensional images Advantages of confocal microscopy - correct answer. increased clarity of images and resolution What is electron microscopy? - correct answer. technique used for obtaining high resolution images of specimens transmission electron microscope - correct answer. used to study the internal structure of cells Advantage of electron microscope - correct answer. shorter wavelength of electron beam= increased resolution and magnification Disadvantage of electron microscopes - correct answer. cannot view living things- life is not compatible with vacuum