MCB Exam 2 - RATs (5-9) UPDATED
Actual Questions and CORRECT Answers
in euk cells the factors that are involved in the most mRNAs are known as - CORRECT
ANSWER- ✔✔general transcription factors
the first phosphorylation event affecting the CTD of the large subunit of ___ is mediated by a
kinase activity assoc. to ___ - CORRECT ANSWER- ✔✔RNA pol II; TFIIH
in euk cells the RNA pol that transcribes messenger RNA is ___ - CORRECT ANSWER-
✔✔RNA pol II
one striking diff at the level in which transcription is initiated btw prok and euk is that -
CORRECT ANSWER- ✔✔in prok all factors required to start transcription are associated to
the holo-enzyme, whereas in euk additional protein factors besides the RNA pol are needed to
initiate transcription
the TFII factor that bridges the interaction btw TFIID to the promoter and positioning it in
place to initiate transcription is ___ - CORRECT ANSWER- ✔✔TFIIB
The TFII factor that contains among its subunits two proteins known as XPD and XPB each
endowed with helicase activity is ___ - CORRECT ANSWER- ✔✔TFIIH
the helicase activity that allows transcription by RNA pol II to start is mediated by -
CORRECT ANSWER- ✔✔TFIIH
evolutionarily speaking the existence in euk of three diff polymerases is most likely the result
of - CORRECT ANSWER- ✔✔a selective pressure to ensure high expression of a specific set
of genes while allowing tight control of others
phosphorylation of the CTD is a key regulatory event during transcriptional initiation by
RNA pol I - CORRECT ANSWER- ✔✔False
, in euk cells transcription of the genes coding for the large ribosomal RNAs yields a large 45S
pre-rRNA that is later on processed to produce the 18S, 5.8S, and 5S rRNAs - CORRECT
ANSWER- ✔✔false
histone deacetylases could be argued to typically ___ gene expression - CORRECT
ANSWER- ✔✔decrease
the essential sequence that drives the recruitment of general transcription factors thus
allowing transcription to occur is known as the - CORRECT ANSWER- ✔✔Core promoter
(?)
these are sequences that divide chromosomes into independent domains and prevent
enhancers form acting on promoters located on a different domain - CORRECT ANSWER-
✔✔insulators
a more ___ chromatin is associated with ___ transcription - CORRECT ANSWER-
✔✔loosely packed; Increased
the helix-turn-helix motifs one example of - CORRECT ANSWER- ✔✔a type of DNA
binding domain
these are activities typically associated to chromatin remodeling factors - CORRECT
ANSWER- ✔✔ATPase, nucleosome eviction, and nucleosome sliding (?)
Sp1 is an example of - CORRECT ANSWER- ✔✔a transcriptional activator
Sp1 is an example of a transcriptional repressor - CORRECT ANSWER- ✔✔False
some euk repressors interfere with the binding of other transcription factors to DNA -
CORRECT ANSWER- ✔✔true
enhancers typically contain only a single sequence element recognized by a single
transcriptional regulatory protein - CORRECT ANSWER- ✔✔false
Actual Questions and CORRECT Answers
in euk cells the factors that are involved in the most mRNAs are known as - CORRECT
ANSWER- ✔✔general transcription factors
the first phosphorylation event affecting the CTD of the large subunit of ___ is mediated by a
kinase activity assoc. to ___ - CORRECT ANSWER- ✔✔RNA pol II; TFIIH
in euk cells the RNA pol that transcribes messenger RNA is ___ - CORRECT ANSWER-
✔✔RNA pol II
one striking diff at the level in which transcription is initiated btw prok and euk is that -
CORRECT ANSWER- ✔✔in prok all factors required to start transcription are associated to
the holo-enzyme, whereas in euk additional protein factors besides the RNA pol are needed to
initiate transcription
the TFII factor that bridges the interaction btw TFIID to the promoter and positioning it in
place to initiate transcription is ___ - CORRECT ANSWER- ✔✔TFIIB
The TFII factor that contains among its subunits two proteins known as XPD and XPB each
endowed with helicase activity is ___ - CORRECT ANSWER- ✔✔TFIIH
the helicase activity that allows transcription by RNA pol II to start is mediated by -
CORRECT ANSWER- ✔✔TFIIH
evolutionarily speaking the existence in euk of three diff polymerases is most likely the result
of - CORRECT ANSWER- ✔✔a selective pressure to ensure high expression of a specific set
of genes while allowing tight control of others
phosphorylation of the CTD is a key regulatory event during transcriptional initiation by
RNA pol I - CORRECT ANSWER- ✔✔False
, in euk cells transcription of the genes coding for the large ribosomal RNAs yields a large 45S
pre-rRNA that is later on processed to produce the 18S, 5.8S, and 5S rRNAs - CORRECT
ANSWER- ✔✔false
histone deacetylases could be argued to typically ___ gene expression - CORRECT
ANSWER- ✔✔decrease
the essential sequence that drives the recruitment of general transcription factors thus
allowing transcription to occur is known as the - CORRECT ANSWER- ✔✔Core promoter
(?)
these are sequences that divide chromosomes into independent domains and prevent
enhancers form acting on promoters located on a different domain - CORRECT ANSWER-
✔✔insulators
a more ___ chromatin is associated with ___ transcription - CORRECT ANSWER-
✔✔loosely packed; Increased
the helix-turn-helix motifs one example of - CORRECT ANSWER- ✔✔a type of DNA
binding domain
these are activities typically associated to chromatin remodeling factors - CORRECT
ANSWER- ✔✔ATPase, nucleosome eviction, and nucleosome sliding (?)
Sp1 is an example of - CORRECT ANSWER- ✔✔a transcriptional activator
Sp1 is an example of a transcriptional repressor - CORRECT ANSWER- ✔✔False
some euk repressors interfere with the binding of other transcription factors to DNA -
CORRECT ANSWER- ✔✔true
enhancers typically contain only a single sequence element recognized by a single
transcriptional regulatory protein - CORRECT ANSWER- ✔✔false
