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Tests for Carbohydrates, Lipids
and Proteins
Tests for carbohydrates:
Benedict’s test- reducing sugars:
Tests for reducing sugars- carbohydrates.
A reducing sugar is a sugar that can give electrons to (reduce) other molecules.
They include all monosaccharides and most disaccharides like maltose and
lactose.
The sample to be tested is placed in a clean test tube
An equal volume of Benedict’s reagent (copper (||) sulfate) is added using a
pipette
Tests for Carbohydrates, Lipids and Proteins 1
, The solution is then heated in a boiling water bath for 3-5 minutes.
If reducing sugars are present, they will reduce the Cu2+ ions in copper (||)
sulfate to Cu+ ions, forming the orange-red precipitate copper (|) oxide.
Tests for Carbohydrates, Lipids and Proteins 2
Tests for Carbohydrates, Lipids
and Proteins
Tests for carbohydrates:
Benedict’s test- reducing sugars:
Tests for reducing sugars- carbohydrates.
A reducing sugar is a sugar that can give electrons to (reduce) other molecules.
They include all monosaccharides and most disaccharides like maltose and
lactose.
The sample to be tested is placed in a clean test tube
An equal volume of Benedict’s reagent (copper (||) sulfate) is added using a
pipette
Tests for Carbohydrates, Lipids and Proteins 1
, The solution is then heated in a boiling water bath for 3-5 minutes.
If reducing sugars are present, they will reduce the Cu2+ ions in copper (||)
sulfate to Cu+ ions, forming the orange-red precipitate copper (|) oxide.
Tests for Carbohydrates, Lipids and Proteins 2