MLT Clinical Chemistry Final Test
with Correct Answers
What is the isoelectric point? - Answer-the pH of a solution where a molecule is neutral
Which of the following methodologies measures absorbed light?
a. liquid chromatography
b. ELISA
c. electrophoresis
d. spectrophotometry - Answer-spectrophotometry
Which of the following is not an amino acid?
a. leucine
b. serine
c. proline
d. theriosine - Answer-theriosine
There are ___________ non-essential and __________ essential amino acids. -
Answer-10, 10
Which essential amino acid helps to induce sleep and is a precursor for serotonin? -
Answer-tryptophan
Which of the following amino acids is found in higher concentration in HIV patients?
a. pyroleucine
b. serine
c. selenocycstine
d. guanine - Answer-selenocycstine
What is the cause of most aminacidpathies? - Answer-inherited genes
What makes an amino acid "essential"? - Answer-it is not made by the body
Homocystine levels are used to assess a patient's risk of what? - Answer-thrombolytic
event
The aminoacidpathy phenylketonuria (PKU) is an inability of the body to metabolize
which amino acid? - Answer-phenylalanine
What is a general treatment method for aminoacidpathies? - Answer-controlled diet
What is a characteristic symptom of isovaleric acadamia? - Answer-stinky, sweaty feet
,Patients suffering from maple syrup urine disease have a build-up of which amino
acids?
a. leucine, isoleucine, and valine
b. serine, tyrosine, and cysteine
c. glycine, alanine, and tryptophan
d. leucine, alanine, and serine - Answer-leucine, isoleucine, and valine
What make and amino acid "non-essential"? - Answer-it is made by the body
Which two aminoacidpathies can lead to the build up of ammonia? - Answer-
Arginosuccinic Aciduria and Citrullemia
Which of the following is an acceptable sample for amino acid analysis?
a. sweat collected in a strerile cup
b. whole blood in an EDTA tube
c. plasma drawn in a sodium heparin tube
d. serum drawn in a marble top tube - Answer-plasma drawn in a sodium heparin tube
What methodology is used to test for amino acids? - Answer-thin-layer chromatography
(TLC) &
high-performance liquid chromatography (HPLC)
Which of the following aminoacidpathies is caused by a cystathionine-Beta-synthase
deficiency:
a. homocysteinuria
b. maple syrup urine disease
c. alkaptonuria
d. PKU - Answer-homocysteinuria
Which aminoacidpathy is not caused by an enzyme deficiency, but is due to problems
with absorption? - Answer-cystineuria
On average how much of a protein's mass is attributed to nitrogen? - Answer-16%
What structural level consists of a(alpha)-helixes and B(beta)-pleasted sheets? -
Answer-secondary
All of the following are functions of proteins except?
a. acid-base balance
b. catalysts
c. energy
d. storage of genetic material - Answer-storage of genetic material
What pH range is required fro proteins in the body to remain soluble? - Answer-7.35-
7.45
, Prealbumin is a carrier protein for what in the serum? - Answer-throxyine
What is the serum protein that is present in the highest concentration? - Answer-
albumin
Elevated levels of a1 (alpha one)- fetoprotein in the fetus are indicative of what
condition? - Answer-spina bifida
Which of the following is not an a2(alpha)-globulin?
a. ceruloplasmin
b. tranferrin
c. haptoglobulin
d. a2(alpha) - Answer-tranferrin
Enzymes that can bind only one substrate and catalyze one reaction exhibit which type
of binding specificity? - Answer-Absolute binding
The rate of an enzymatic reaction will ___________________ for every 10 degrees C
temperature increase until the enzyme denatures. - Answer-Double
All of the following conditions may result in a rise in CKBB levels except.
a. Child birth
b Spinal chord injury
c. Myocardial infarction
d. Brain tumor - Answer-c. Myocardial infarction
Which two enzymes will show significant elevations with viral hepatitis? - Answer-AST &
ALT
GGT is an enzyme that can be used to help monitor the treatment of which patient
population? - Answer-recovering alcoholics
Digestive enzymes circulate in inactive forms called __________________. - Answer-
Zymogens
A patient comes to the ER with abdominal pain, the physician ordered an amylase,
lipase, and lipid panel. The triglyceride result is significantly elevated, how might this
effect the amylase and lipid levels? (thrown out) - Answer-Unchanged lipase, decreased
amylase
Which of the following is a function of glucose-6-phosphate dehydrogenase (G6PD)
a. prevents RBC lysis
b. inhibits CK
c. stimulates insulin secretion
d. catalyzes the conversion of glutamate - Answer-a. prevents RBC lysis
with Correct Answers
What is the isoelectric point? - Answer-the pH of a solution where a molecule is neutral
Which of the following methodologies measures absorbed light?
a. liquid chromatography
b. ELISA
c. electrophoresis
d. spectrophotometry - Answer-spectrophotometry
Which of the following is not an amino acid?
a. leucine
b. serine
c. proline
d. theriosine - Answer-theriosine
There are ___________ non-essential and __________ essential amino acids. -
Answer-10, 10
Which essential amino acid helps to induce sleep and is a precursor for serotonin? -
Answer-tryptophan
Which of the following amino acids is found in higher concentration in HIV patients?
a. pyroleucine
b. serine
c. selenocycstine
d. guanine - Answer-selenocycstine
What is the cause of most aminacidpathies? - Answer-inherited genes
What makes an amino acid "essential"? - Answer-it is not made by the body
Homocystine levels are used to assess a patient's risk of what? - Answer-thrombolytic
event
The aminoacidpathy phenylketonuria (PKU) is an inability of the body to metabolize
which amino acid? - Answer-phenylalanine
What is a general treatment method for aminoacidpathies? - Answer-controlled diet
What is a characteristic symptom of isovaleric acadamia? - Answer-stinky, sweaty feet
,Patients suffering from maple syrup urine disease have a build-up of which amino
acids?
a. leucine, isoleucine, and valine
b. serine, tyrosine, and cysteine
c. glycine, alanine, and tryptophan
d. leucine, alanine, and serine - Answer-leucine, isoleucine, and valine
What make and amino acid "non-essential"? - Answer-it is made by the body
Which two aminoacidpathies can lead to the build up of ammonia? - Answer-
Arginosuccinic Aciduria and Citrullemia
Which of the following is an acceptable sample for amino acid analysis?
a. sweat collected in a strerile cup
b. whole blood in an EDTA tube
c. plasma drawn in a sodium heparin tube
d. serum drawn in a marble top tube - Answer-plasma drawn in a sodium heparin tube
What methodology is used to test for amino acids? - Answer-thin-layer chromatography
(TLC) &
high-performance liquid chromatography (HPLC)
Which of the following aminoacidpathies is caused by a cystathionine-Beta-synthase
deficiency:
a. homocysteinuria
b. maple syrup urine disease
c. alkaptonuria
d. PKU - Answer-homocysteinuria
Which aminoacidpathy is not caused by an enzyme deficiency, but is due to problems
with absorption? - Answer-cystineuria
On average how much of a protein's mass is attributed to nitrogen? - Answer-16%
What structural level consists of a(alpha)-helixes and B(beta)-pleasted sheets? -
Answer-secondary
All of the following are functions of proteins except?
a. acid-base balance
b. catalysts
c. energy
d. storage of genetic material - Answer-storage of genetic material
What pH range is required fro proteins in the body to remain soluble? - Answer-7.35-
7.45
, Prealbumin is a carrier protein for what in the serum? - Answer-throxyine
What is the serum protein that is present in the highest concentration? - Answer-
albumin
Elevated levels of a1 (alpha one)- fetoprotein in the fetus are indicative of what
condition? - Answer-spina bifida
Which of the following is not an a2(alpha)-globulin?
a. ceruloplasmin
b. tranferrin
c. haptoglobulin
d. a2(alpha) - Answer-tranferrin
Enzymes that can bind only one substrate and catalyze one reaction exhibit which type
of binding specificity? - Answer-Absolute binding
The rate of an enzymatic reaction will ___________________ for every 10 degrees C
temperature increase until the enzyme denatures. - Answer-Double
All of the following conditions may result in a rise in CKBB levels except.
a. Child birth
b Spinal chord injury
c. Myocardial infarction
d. Brain tumor - Answer-c. Myocardial infarction
Which two enzymes will show significant elevations with viral hepatitis? - Answer-AST &
ALT
GGT is an enzyme that can be used to help monitor the treatment of which patient
population? - Answer-recovering alcoholics
Digestive enzymes circulate in inactive forms called __________________. - Answer-
Zymogens
A patient comes to the ER with abdominal pain, the physician ordered an amylase,
lipase, and lipid panel. The triglyceride result is significantly elevated, how might this
effect the amylase and lipid levels? (thrown out) - Answer-Unchanged lipase, decreased
amylase
Which of the following is a function of glucose-6-phosphate dehydrogenase (G6PD)
a. prevents RBC lysis
b. inhibits CK
c. stimulates insulin secretion
d. catalyzes the conversion of glutamate - Answer-a. prevents RBC lysis
