Protein Electrophoresis (Clinical Chemistry) – Q’s & A’s
Which of the following proteins migrates in the Alpha2-globulin region at pH
8.6?
A) transferrin
B) haptoglobin
C) hemopexin
D) fibrinogen Right Ans - B
An electrophoretic separation of lactate dehydrogenase isoenzyme that
demonstrates elevation in LD-1 greater than LD-2 could be indicative of:
A) A normal LD isoenzyme pattern
B) Hemolysis
C) Pancreatitis
D) Hepatic injury Right Ans - B) Hemolysis
Hemolysis, both in vivo and in vitro, can cause elevations in LD-1 and LD-2.
Also, LD-1 and LD-2 are both increased during myocardial infarction since
they are both present in heart muscle.
In normal conditions, LD-2 is present in higher concentrations than LD-1.
The reverse is true in myocardial infarction and during states of hemolysis.
This term is referred to as the LD flip.
Pancreatitis does cause increased levels of LD, but the LD-3 type is the most
affected in this condition.Hepatic injury also increases levels of LD, but the LD-
4 type is the most affected in this condition.
Elevated serum protein and decreased albumin are associated with:
A) Glomerulonephritis
B) cirrhosis
C) Starvation/anorexia
D) Multiple Myeloma Right Ans - D
, Which statement is correct for a protein with an isoelectric point (pI) of 7.0?
A) At pH < 7.0, this protein will migrate to the anode.
B) At pH < 7.0, this protein will be positively charged.
C) At pH < 7.0, this protein will be negatively charged.
D) At pH > 7.0, this protein will migrate to the cathode. Right Ans - B) At pH
< 7.0, this protein will be positively charged.
The isoelectric point (pI) of a protein is the pH where the net charge is zero.
This means that the protein has an equal number of groups with a positive
charge and groups with a negative charge.
Charged particles in an electrical field will migrate to the electrode of the
opposite charge.
When the pH is less than the pI, the protein has a net positive charge (cation)
and will migrate to the cathode (electrode with a negative charge).
When the pH is greater than the pI, the protein has a net negative charge
(anion) and will migrate to the anode (electrode with a positive charge).
The screening test for antenatal detection of nueral tube defects is:
A) Alpha fetoprotein
B) ceruloplasmin
C) Beta2-microglobulin
D) Alpha1-antichymotrypsin Right Ans - A
Which of the following conditions is associated with an acute inflammatory
pattern on protein electrophoresis?
A) Cirrhosis
B) Rheumatoid arthritis
C) Myocardial infarction
Which of the following proteins migrates in the Alpha2-globulin region at pH
8.6?
A) transferrin
B) haptoglobin
C) hemopexin
D) fibrinogen Right Ans - B
An electrophoretic separation of lactate dehydrogenase isoenzyme that
demonstrates elevation in LD-1 greater than LD-2 could be indicative of:
A) A normal LD isoenzyme pattern
B) Hemolysis
C) Pancreatitis
D) Hepatic injury Right Ans - B) Hemolysis
Hemolysis, both in vivo and in vitro, can cause elevations in LD-1 and LD-2.
Also, LD-1 and LD-2 are both increased during myocardial infarction since
they are both present in heart muscle.
In normal conditions, LD-2 is present in higher concentrations than LD-1.
The reverse is true in myocardial infarction and during states of hemolysis.
This term is referred to as the LD flip.
Pancreatitis does cause increased levels of LD, but the LD-3 type is the most
affected in this condition.Hepatic injury also increases levels of LD, but the LD-
4 type is the most affected in this condition.
Elevated serum protein and decreased albumin are associated with:
A) Glomerulonephritis
B) cirrhosis
C) Starvation/anorexia
D) Multiple Myeloma Right Ans - D
, Which statement is correct for a protein with an isoelectric point (pI) of 7.0?
A) At pH < 7.0, this protein will migrate to the anode.
B) At pH < 7.0, this protein will be positively charged.
C) At pH < 7.0, this protein will be negatively charged.
D) At pH > 7.0, this protein will migrate to the cathode. Right Ans - B) At pH
< 7.0, this protein will be positively charged.
The isoelectric point (pI) of a protein is the pH where the net charge is zero.
This means that the protein has an equal number of groups with a positive
charge and groups with a negative charge.
Charged particles in an electrical field will migrate to the electrode of the
opposite charge.
When the pH is less than the pI, the protein has a net positive charge (cation)
and will migrate to the cathode (electrode with a negative charge).
When the pH is greater than the pI, the protein has a net negative charge
(anion) and will migrate to the anode (electrode with a positive charge).
The screening test for antenatal detection of nueral tube defects is:
A) Alpha fetoprotein
B) ceruloplasmin
C) Beta2-microglobulin
D) Alpha1-antichymotrypsin Right Ans - A
Which of the following conditions is associated with an acute inflammatory
pattern on protein electrophoresis?
A) Cirrhosis
B) Rheumatoid arthritis
C) Myocardial infarction
