Case 3
Learning goals
1. Translation
2. Structure of ribosome
3. Structure, lifetime and degradation of a protein
4. Protein modification
5. How is the protein synthesis regulated?
, 1. Translation
Initiation (prokaryotes)
Shine-Dalgarno sequence: important for the small ribosomal subunit
(30S ribosomal complex) to attach to the mRNA
happens through 16S-rRNA
In the beginning a (f)Met (UAC) bonds to the startcodon (AUG).
(In eukaryotes it’s just Met)
Then the large ribosomal unit (50S ribosomal complex) attaches to the
whole system. In such a way that (f)Met is on the P-site.
- A-site: arrival
- P-site: protein
- E-site: exit
When you release the initiation factors and you hydrolyse the GDP + P
GTP:
70S initiation complex.
But 30S + 50S = 80. While the complex has 70S.
S stands for Svedbergs = a unit used to measure how fast molecules
move in a centrifuge. But the 30S is not entirely 30 and so is the 50S.
That’s why it’s more 70S than 80S.
Learning goals
1. Translation
2. Structure of ribosome
3. Structure, lifetime and degradation of a protein
4. Protein modification
5. How is the protein synthesis regulated?
, 1. Translation
Initiation (prokaryotes)
Shine-Dalgarno sequence: important for the small ribosomal subunit
(30S ribosomal complex) to attach to the mRNA
happens through 16S-rRNA
In the beginning a (f)Met (UAC) bonds to the startcodon (AUG).
(In eukaryotes it’s just Met)
Then the large ribosomal unit (50S ribosomal complex) attaches to the
whole system. In such a way that (f)Met is on the P-site.
- A-site: arrival
- P-site: protein
- E-site: exit
When you release the initiation factors and you hydrolyse the GDP + P
GTP:
70S initiation complex.
But 30S + 50S = 80. While the complex has 70S.
S stands for Svedbergs = a unit used to measure how fast molecules
move in a centrifuge. But the 30S is not entirely 30 and so is the 50S.
That’s why it’s more 70S than 80S.
