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HDWK Ch. 5 Activity Fig. 5.8 Questions and Answers | Latest Update | 2024/2025 | Already Passed

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HDWK Ch. 5 Activity Fig. 5.8 Questions and Answers | Latest Update | 2024/2025 | Already Passed **Why did researchers attach a fluorescent dye to membrane proteins?** The fluorescent dye was attached to enable visualization of proteins within the membrane plane. **Following laser application, the bleached spot gradually regains fluorescence over time. What conclusion did researchers draw from this observation?** Researchers concluded that proteins exhibit movement within the membrane plane. **What does the y-axis indicate in the graph?** It represents the fluorescence level in the bleached area of the cell. **Why does the fluorescence level decrease significantly during the experiment?** This decrease occurs because a laser is used to bleach a specific area of the membrane. **If proteins were not labeled with a fluorescent dye, how would the graph appear?** *There would be no observable change in fluorescence over time.* **To investigate whether phospholipids move within the membrane plane, what approach could researchers take?** Researchers could conduct a similar experiment but label phospholipids with a fluorescent dye instead of proteins. **While many proteins move freely within the membrane plane, some are confined to specific regions. If a membrane protein restricted to a specific region were labeled in a FRAP experiment, what result might be observed?** The bleached spot would not recover its fluorescence over time. **Scientists often employ multiple techniques to validate their findings. In a related experiment, mouse and human cells were fused,

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