Blood Bank Review
Describe antigen development in a newborn - CORRECT ANSWER-ABO
antigens are weaker in cord blood samples and may result in weaker ABO
phenotyping reactions.
Antigen development slowly occurs until the full expression of adult levels is
reached at about 2 to 4 years of age.
Sugar for H angtigen - CORRECT ANSWER-L-fucose
Genetic control of A and B antigens is mapped to chromosome ___ - CORRECT
ANSWER-9
H antigens by phenotype (highest to lowest concentration - CORRECT
ANSWER-O>A2>B>A2B>A1>A1B
Which phenotype possesses the lowest number of unconverted H sites -
CORRECT ANSWER-Group A1B
The anti-A1 lectin is______ in the routine ABO testing of donors and recipients
because it is unnecessary to distinguish between the A1 and A2 phenotypes for
transfusion purposes. - CORRECT ANSWER-not used
Adsorption - CORRECT ANSWER-immunohematologic technique that uses red
cells (known antigens) to remove red cell antibodies from a solution (plasma or
antisera); group A red cells can remove anti-A from solution.
Elution - CORRECT ANSWER-process that dissociates antigen-antibody
complexes on red cells; freed IgG antibody is tested for specificity.
Ulex europaeus - CORRECT ANSWER-Plant lectin with specificity for the H
antigen
ABO antibodies are not detected in the serum of newborns until they are _____ -
CORRECT ANSWER-3 to 6 months of age
, Maximal ABO titers have been reported in children from __________ -
CORRECT ANSWER-5 to 10 years of age.
The anti-A produced in group B individuals and the anti-B produced in group A
individuals contain primarily antibodies of the ___ - CORRECT
ANSWER-immunoglobulin M (IgM) immunoglobulin class along with small
amounts of IgG.
Anti-A and anti-B antibodies found in the serum of group O individuals are
composed - CORRECT ANSWER-primarily of IgG class.
Anti-A1 becomes a concern when it causes problems with ABO phenotyping
results and incompatible crossmatches on immediate spin. - CORRECT
ANSWER-...
Red cell suspensions prepared from patient samples can be washed three times
before repeated testing - CORRECT ANSWER-...
Daily quality control on ABO typing reagents is satisfactory - CORRECT
ANSWER-...
Acquired B phenotype - CORRECT ANSWER-group A1 individual with disease
of the lower gastrointestinal tract, cancer of the colon and rectum, intestinal
obstruction, or gram-negative septicemia who acquires reactivity with anti-B
reagents in ABO red cell testing and appears as group AB.
B(A) phenotype - CORRECT ANSWER-group B individual who acquires
reactivity with anti-A reagents in ABO red cell testing; in these individuals the B
gene transfers trace amounts of the immunodominant sugar for the A antigen
and the immunodominant sugar for the B antigen
Acquired B phenotype - CORRECT ANSWER-1. The agglutination of the
patient's red cells with anti-A is strong (4+).
2. The agglutination of the patient's red cells with anti-B is weaker (1+) than
usually expected (3+ to 4+). These red cells react as the phenotype group AB.
Describe antigen development in a newborn - CORRECT ANSWER-ABO
antigens are weaker in cord blood samples and may result in weaker ABO
phenotyping reactions.
Antigen development slowly occurs until the full expression of adult levels is
reached at about 2 to 4 years of age.
Sugar for H angtigen - CORRECT ANSWER-L-fucose
Genetic control of A and B antigens is mapped to chromosome ___ - CORRECT
ANSWER-9
H antigens by phenotype (highest to lowest concentration - CORRECT
ANSWER-O>A2>B>A2B>A1>A1B
Which phenotype possesses the lowest number of unconverted H sites -
CORRECT ANSWER-Group A1B
The anti-A1 lectin is______ in the routine ABO testing of donors and recipients
because it is unnecessary to distinguish between the A1 and A2 phenotypes for
transfusion purposes. - CORRECT ANSWER-not used
Adsorption - CORRECT ANSWER-immunohematologic technique that uses red
cells (known antigens) to remove red cell antibodies from a solution (plasma or
antisera); group A red cells can remove anti-A from solution.
Elution - CORRECT ANSWER-process that dissociates antigen-antibody
complexes on red cells; freed IgG antibody is tested for specificity.
Ulex europaeus - CORRECT ANSWER-Plant lectin with specificity for the H
antigen
ABO antibodies are not detected in the serum of newborns until they are _____ -
CORRECT ANSWER-3 to 6 months of age
, Maximal ABO titers have been reported in children from __________ -
CORRECT ANSWER-5 to 10 years of age.
The anti-A produced in group B individuals and the anti-B produced in group A
individuals contain primarily antibodies of the ___ - CORRECT
ANSWER-immunoglobulin M (IgM) immunoglobulin class along with small
amounts of IgG.
Anti-A and anti-B antibodies found in the serum of group O individuals are
composed - CORRECT ANSWER-primarily of IgG class.
Anti-A1 becomes a concern when it causes problems with ABO phenotyping
results and incompatible crossmatches on immediate spin. - CORRECT
ANSWER-...
Red cell suspensions prepared from patient samples can be washed three times
before repeated testing - CORRECT ANSWER-...
Daily quality control on ABO typing reagents is satisfactory - CORRECT
ANSWER-...
Acquired B phenotype - CORRECT ANSWER-group A1 individual with disease
of the lower gastrointestinal tract, cancer of the colon and rectum, intestinal
obstruction, or gram-negative septicemia who acquires reactivity with anti-B
reagents in ABO red cell testing and appears as group AB.
B(A) phenotype - CORRECT ANSWER-group B individual who acquires
reactivity with anti-A reagents in ABO red cell testing; in these individuals the B
gene transfers trace amounts of the immunodominant sugar for the A antigen
and the immunodominant sugar for the B antigen
Acquired B phenotype - CORRECT ANSWER-1. The agglutination of the
patient's red cells with anti-A is strong (4+).
2. The agglutination of the patient's red cells with anti-B is weaker (1+) than
usually expected (3+ to 4+). These red cells react as the phenotype group AB.
