BIO 171- Microbiology Lab Notebook
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Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with
how to conduct experiments and practice proper safety procedures
while in a Lab. Cultivation, Identification, and Evaluation are the 3 main
principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of
microbiology lab with an introduction to basic equipment and safety
rules. We are also show how to keep our Lab Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone
healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer
machine with a thick door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
, ▪ Put tools in before you use for a lab OR put
contaminated tools to kill bacteria
▪ Not just dry air, steam is more efficient
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
, ➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes
and rotates culture in a swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve
samples and keep them for long term.
• Test tubes which may have liquid broth, petri dishes which
are wrapped airtight, or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
Latest Updated 2024 Guaranteed
Success A+
Lab Notebook Bookmarks (click to
navigate): Lab 1
Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
, Lab 1
Notebook
Back to Home
Page
Title: Introduction to Medical Microbiology
Objective: The objective of this lab will be to familiarize myself with
how to conduct experiments and practice proper safety procedures
while in a Lab. Cultivation, Identification, and Evaluation are the 3 main
principles discussed when conducting any experiment.
Procedure: Our instructor introduces us to the foundation of
microbiology lab with an introduction to basic equipment and safety
rules. We are also show how to keep our Lab Notebook formatted.
Notes: Basic Equipment
❖ Cleaning – need to work in a sterile environment to keep everyone
healthy
o Main tool: Autoclave 125 degrees C (looks like a giant dryer
machine with a thick door)
▪ Come in variety of sizes
▪ Uses heat, pressure, and steam to sterilize environment
, ▪ Put tools in before you use for a lab OR put
contaminated tools to kill bacteria
▪ Not just dry air, steam is more efficient
❖ Growing- how to grow the pathogens and bacteria?
❖ Two Main Ways:
, ➢ Fixed incubator 37 degrees C (Chamber- looks like a mini fridge)
▪ Stored Petri dishes, which contain the samples
▪ Airtight seal, and metal door to keep dark conditions
➢ Shaker incubator 37 degrees C
▪ Used to take a growth media, which is liquid nutrient broth
▪ After inoculating bacteria, put it in here, and shaker shakes
and rotates culture in a swirling fashion
▪ Helps arete it and help bacteria grow under optimal conditions
❖ Visualizing- How do we see these bacteria and things?
➢ Microscopy
▪ Beneficial to seeing bacteria
❖ Storing
➢ Refrigerator 4 degree C
▪ Temperature slows bacterial growth, which helps preserve
samples and keep them for long term.
• Test tubes which may have liquid broth, petri dishes which
are wrapped airtight, or test tube racks
General Lab Safety
❖ Never eat or drink in lab
➢ Contamination risks
❖ Use PPE
