Bio 250 Midterm Questions with Correct Answers

Immunochemistry Correct Answer -used to find antigens, proteins, and cancer in specific tissues via antibodies Induced pluripotent stem-cell programming Correct Answer -tweaks adult cells into stem cells to force them to express genes, to have properties of embryonic stem cells, to model disease and study drug development RT-qpcr Correct Answer -used to analyze gene expression (how much is gene expressed?) Real time PCR Correct Answer -measures telomere length in humans Western blot Correct Answer -detects presence of specific proteins in a tissue sample Transfection Correct Answer -gene control/silencing -virus production -protein production -differentiation Viral vector Correct Answer -insertion of genes -over expression of genes Cre-lox Correct Answer -deletions, insertions, translations and inversions of specific sites of DNA How is cre-lox different from viral vectors, transfection or rnai? Correct Answer -broader manipulation of genome Sandwich ELISA Correct Answer -tests presence of antigens or antibodies -measure concentration of molecules and proteins Rnai Correct Answer -used to knockout genes at a translational level Northern blot Correct Answer -studies gene expression via detection of RNA -determines the presence of an expressed gene in a sample