TLC exams with all corect answers 2024

What is one of the most critical aspects of TLC correct answersFinding a good solvent system The Six Steps of TLC correct answersStep 1. Preparing the Sample Step 2. Spotting the TLC Plate Step 3. Picking a Solvent Step 4. Developing the Plate Step 5. Visualizing the Results Step 6. Calculating Rf Values Step 1: When preparing the sample correct answers What will happen if the prepared sample is too concentrated? correct answers What does streaking lead to? correct answers Step 2: Spotting the TLC plate correct answersSpotting can be made by using capillary beds 1mm What will happen if the spots are too large? correct answersThen two or more spots of a sample may overlap on the TLC plate, thus causing erroneous conclusions about the separation and/or the sample's purity or content. Step 3: Picking the solvent correct answersOne of the most crucial parts of a successful TLC separation is choosing the appro- priate mobile phase; see the section "Mobile Phase Polarity". Step 4: Developing the Plate. How does the solvent travel up? correct answers Step 5: Visualizing the Results. Why use UV light to observe the separated spots? correct answersUV light works well for observing the separated spots. TLC plates normally contain a fluorescent indicator that makes them glow green under UV light of wavelength 254 nm.