QBM Practice Exam 4 ( Questions And Answers) Verified
100% Correct!!
Which of the following expression systems is fastest, but would not glycosylate a
protein?
Bacteria
Transfection
can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-
translated double-stranded RNA (dsRNA) molecules called small interfering RNA
(siRNA) mediate sequence specific degradation of target messenger RNA
(mRNA).
RNA interference
Cells can be directly modified via __________, which involves using a small glass
pipette or microscopic lance to add or remove nuclear material (enucleation) or
inject molecules into a cell, through the plasma membrane.
microinjection
Immunofluorescence is most similar to performing
a western blot.
Which of the following techniques would be best suited to directly study cellular
metabolism in vitro?
Pulse-chase.
In FACS, cells can be separated based on
stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the
side scatter indicates __________.
the size of a cell ; the cell's granularity, or health
During in situ organ perfusion,
an organ is removed from the model organism, maintained at constant temperature and
pH in an apparatus, and fluid is passed through the organ.
Which of the following is a benefit of lower-order model organisms versus higher-
order model organisms?
Short development time.
Any investigator wishing to use animals in a research program must first submit
a detailed animal protocol to __________ for approval.
the IACUC committee
Which of the following statements concerning transgenic animals is correct?
Pigs with human-sized organs have been designed, to aid in the supply of transplant
organs.
The incorporation of GFP into the genome of animals results in the creation of a
transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by
100% Correct!!
Which of the following expression systems is fastest, but would not glycosylate a
protein?
Bacteria
Transfection
can be performed using the capabilities of retroviruses.
__________ is a form of post-transcriptional gene regulation in which non-
translated double-stranded RNA (dsRNA) molecules called small interfering RNA
(siRNA) mediate sequence specific degradation of target messenger RNA
(mRNA).
RNA interference
Cells can be directly modified via __________, which involves using a small glass
pipette or microscopic lance to add or remove nuclear material (enucleation) or
inject molecules into a cell, through the plasma membrane.
microinjection
Immunofluorescence is most similar to performing
a western blot.
Which of the following techniques would be best suited to directly study cellular
metabolism in vitro?
Pulse-chase.
In FACS, cells can be separated based on
stage of cell cycle.
size.
health.
number of chromosomes.
In flow cytometry, the forward scattering of light can indicate __________, and the
side scatter indicates __________.
the size of a cell ; the cell's granularity, or health
During in situ organ perfusion,
an organ is removed from the model organism, maintained at constant temperature and
pH in an apparatus, and fluid is passed through the organ.
Which of the following is a benefit of lower-order model organisms versus higher-
order model organisms?
Short development time.
Any investigator wishing to use animals in a research program must first submit
a detailed animal protocol to __________ for approval.
the IACUC committee
Which of the following statements concerning transgenic animals is correct?
Pigs with human-sized organs have been designed, to aid in the supply of transplant
organs.
The incorporation of GFP into the genome of animals results in the creation of a
transgenic animal.
Transgenic animals have been used to make model organisms for human illnesses, by
