Nam Megan Thurmon
ENZYME REPORT SHEET e:
Unit
#
Download the report sheet, complete it,
and then submit it via Blackboard email as an attachment. Do not change
the format.
All blue shaded areas require answers.
Introduction: Catechol oxidase reaction
Name of substrates Catechol Oxygen
Name of enzyme Catechole oxidase
Name of products benzoquinone Water
Enzyme was extracted from Potato extract
Color of benzoquinone orange
m
er as
co
eH w
I. Preparation of standard tubes
Experiment (dry lab)
o.
rs e
ou urc
Copy the color intensity for the 20 min standard tubes from the
slide.
Time (min) Tube A Tube B Tube C
o
20 0 0 5
aC s
vi y re
II. Specificity
Experiment (dry lab)
ed d
Record intensity (scale 0 – 5) using the standards (A-C) as your
ar stu
guide
Time (min) Tube D Tube E
20 orange Yellow-
orange
is
Th
1. Based on the color intensity of the tubes, which Tube
substrate reacts better with catechol oxidase to produce D
benzoquinone?
sh
Choices are catechol (tube D) or hydroquinone (tube E).
Yes
2. Does catechol oxidase express specificity? Yes or No.
This study source was downloaded by 100000829907498 from CourseHero.com on 08-10-2021 17:21:02 GMT -05:00
https://www.coursehero.com/file/61079761/enzymes-report-sheetdocx/
, III. Temperature
At extremely high temperatures and pH, the structure of the Denatured
enzyme is permanently changed to the point where it can no
longer function. This change in structure of the enzyme is
described as
Experiment (dry lab)
Record the color of each tube at 20 minutes on a scale of 0-5 using your
standards (A-C) as a guide. Plot your results on the graph below and connect
the dots.
Time 50C 250C 400C 600C 800C 1000C
(min) ice water room temp Water Water Water Boiling
water bath bath bath water
m
er as
20 yellow orange red orange yellow clear
co
eH w
40 Degrees
At what temperature does catechol oxidase work best? C
o.
rs e
ou urc
o
aC s
vi y re
ed d
ar stu
is
Th
sh
This study source was downloaded by 100000829907498 from CourseHero.com on 08-10-2021 17:21:02 GMT -05:00
https://www.coursehero.com/file/61079761/enzymes-report-sheetdocx/
ENZYME REPORT SHEET e:
Unit
#
Download the report sheet, complete it,
and then submit it via Blackboard email as an attachment. Do not change
the format.
All blue shaded areas require answers.
Introduction: Catechol oxidase reaction
Name of substrates Catechol Oxygen
Name of enzyme Catechole oxidase
Name of products benzoquinone Water
Enzyme was extracted from Potato extract
Color of benzoquinone orange
m
er as
co
eH w
I. Preparation of standard tubes
Experiment (dry lab)
o.
rs e
ou urc
Copy the color intensity for the 20 min standard tubes from the
slide.
Time (min) Tube A Tube B Tube C
o
20 0 0 5
aC s
vi y re
II. Specificity
Experiment (dry lab)
ed d
Record intensity (scale 0 – 5) using the standards (A-C) as your
ar stu
guide
Time (min) Tube D Tube E
20 orange Yellow-
orange
is
Th
1. Based on the color intensity of the tubes, which Tube
substrate reacts better with catechol oxidase to produce D
benzoquinone?
sh
Choices are catechol (tube D) or hydroquinone (tube E).
Yes
2. Does catechol oxidase express specificity? Yes or No.
This study source was downloaded by 100000829907498 from CourseHero.com on 08-10-2021 17:21:02 GMT -05:00
https://www.coursehero.com/file/61079761/enzymes-report-sheetdocx/
, III. Temperature
At extremely high temperatures and pH, the structure of the Denatured
enzyme is permanently changed to the point where it can no
longer function. This change in structure of the enzyme is
described as
Experiment (dry lab)
Record the color of each tube at 20 minutes on a scale of 0-5 using your
standards (A-C) as a guide. Plot your results on the graph below and connect
the dots.
Time 50C 250C 400C 600C 800C 1000C
(min) ice water room temp Water Water Water Boiling
water bath bath bath water
m
er as
20 yellow orange red orange yellow clear
co
eH w
40 Degrees
At what temperature does catechol oxidase work best? C
o.
rs e
ou urc
o
aC s
vi y re
ed d
ar stu
is
Th
sh
This study source was downloaded by 100000829907498 from CourseHero.com on 08-10-2021 17:21:02 GMT -05:00
https://www.coursehero.com/file/61079761/enzymes-report-sheetdocx/
