AAB Molecular Diagnostics Testing

AAB Molecular Diagnostics Testing This DNA amplification method amplifies the nucleic acid used as a probe? - ANSWER Ligase Chain Reaction (LCR) What enzymes are used in Ligase Chain Reactions? - ANSWER DNA polymerase and DNA ligase What is the function of DNA polymerase in Ligase Chain Reaction? - ANSWER Is used to initiate DNA polymerase. What is the key advantage of using Ligase Chain Reaction instead of Polymerase Chain Reaction? - ANSWER Better specificity How does Ligase Chain Reaction differ from Polymerase Chain Reaction? - ANSWER This method uses a thermostable ligase to join to probes which are then amplified by standard PCR cycling. Why was Ligase Chain Reaction originally developed? - ANSWER To detect point mutation. What do positive controls ensure during PCR testing? - ANSWER That the enzyme is active, the buffer is optimal, the primers are priming the correct sequence, and that the thermal cycler is working properly. What is a reagent blank control in PCR? - ANSWER A negative control that lacks DNA to ensure that the reaction mix is not contaminated with DNA template or amplified products from a previous run. What is a negative template control in PCR? - ANSWER A negative control that contains DNA that lacks this target DNA sequence. This ensures that the primers are not annealing to unintended DNA sequences