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Consider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 - ANSWER 12.42 ( CPI is calculated by multiplying all Paternity index tgt) Best way to avoid PCR contamination - ANSWER Separate Pre-PCR (clean area) and post PCR (dirty area) A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA - ANSWER Protein contamination . A260/280 = 1.3 1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA Why is Fluorometry a better technique that Spectroscopy - ANSWER Increased sensitivity and more importantly can accurately measure DNA concentration of a structurally intact double stranded DNA molecule Linkage Disequilibrium - ANSWER LD is the non random association of alleles at 2 or more loci in a general population. When alleles are in LD, haplotypes do not occur at the expected frequencies. Specificity - ANSWER Specificity = TN / (TN + FP) Limit of Detection (LOD) - ANSWER lowest concentration of analyte that can be detected mRNA Sequence provided - Convert it into DNA - ANSWER (Template/Anti-sense Strand) [Replace U with T] Correct order of HIV Sample Preparation Workflow - ANSWER Plasma Preparation RNA Extraction RNA Amplification/Modification Procedure If a flask of cells is provided for the intended purpose of DNA isolation, how would you proceed further - ANSWER Trypsinize and/or scrape the cells inside the provided flask, collect the cells suspended in the media, spin, and then proceed further Which end of the primer is critical for the increased specification of PCR Amplification process - ANSWER 3'End of the Primer Primer Dimer Complementarity - ANSWER 5' End of the Primers (3-3' Was not an option) Mechanism of Action of MecA Gene in MRSA - ANSWER Altered Drug Target (PBP2A) Technique to be used for FFPE Cervical Tissue Provided - ANSWER Invader Assay (Non-Amplification Method) Liquid Based-Cytology Useful for what kind of virus - ANSWER HPV is the correct answer (Options Provided for: HIV, HBV, HCV, HPV) Isothermal Method that utilizes Primes and Polymerase to Create Synthetic dsDNA - ANSWER SDA (Strand Displacement Amplification) Polymerase used in Isothermal LAMP Procedure - ANSWER BST (Bacillus Stearothermophilus) BRAF V600E Mutation is Associated with - ANSWER Lynch Syndrome (Also Known as HNPCC) CAP Requires Discontinued SOP/Procedures to be stored for how long - ANSWER At least 2 years from the date of discontinuation CAP Requires Laboratory Personnel (Technologist) Proficiency Testing - ANSWER Once a Year Most Important Component of Clinical Laboratory Completed Validation Studies - ANSWER Final Test Validation Summary Signed off by Laboratory Director STR Electropherogram provided and select the most informative loci - ANSWER Two different alleles each was both recipient vs donor the most informative one STR makes use of which of the following - ANSWER Variable Nucleotide Experiment Performed (RFLP + PCR + Gel Electrophoresis): three bands observed in the mutant sequence as compared to the normal sequence - ANSWER Insertion of New Restriction Site Mutation in what gene decreases metabolism of Irinotecan Drug - ANSWER UGT1A1 CYP2D6 Enzyme Metabolizes what drug - ANSWER Codeine Determine Melting Temperature of a given DNA Sequence - ANSWER 2(A+T) + 4(G+C) Specimen Collection for Testing of Cystic Fibrosis - ANSWER Either 1 ml EDTA Lavender Tube/5 ml ACD Tube (Not Sure: I think I answered 1 ml EDTA, however the volume seems to be too low) Fish is used in - ANSWER Prader Willi Syndrome Defects associated with Prader Willi Syndrome - ANSWER Uniparental Disomy and Genomic Imprinting - Paternal Chromsome Deletion 15 Which of the Following is Trinucleotide Repeat Expansion Disorder - ANSWER Huntington's Disease, Fragile X HER2/EGFR2 Overexpression - ANSWER Trastuzumab Therapy Overexpression of estrogen, so treat with Herceptin/Tamoxifin Trastuzumab therapy T-Cell Receptor Gene Rearrangement - ANSWER Hodgkin Lymphoma (PS: Don't get confused with T-Cell Lymphoma that corresponds to Sezary Syndrome, both options will be present for confusion) Genes Involved in the Cell Cycle Process are termed as - ANSWER Cycle-Dependent