Portage Learning microbiology module 3 Exam 2024

Portage Learning microbiology module 3 Exam 2024 A nanometer is defined as: -Answer-10-9 A nanometer is defined as one-billionth of a meter True or False: A nanometeris longer than a micrometer -Answer-False. A nanometer is 1,000 times smaller than a micrometer. Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. -Answer-Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low resolution means two (or more) objects may appear as one. *Contrast is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark objects = high contrast). Assuming a constant (non-adjustable) light source power, identify the part of the microscope you would adjust to limit the amount of light entering the microscope. - Answer-The iris controls the amount of light that passes through the sample and into the objective lens. Thus, it can be adjusted (opened or closed) to alter the amount of light. What is the total magnification (relative to your eye) of a sample imaged with a 60x objective and a 10x eyepiece? Show your math -Answer-60 x 10 = 600x magnification True or False: Staining is often required to image a cell that is adherent and flat (thin). - Answer-True. Adherent, flat and unstained cells are almost invisible due to the limits on both resolution and contrast. Therefore, cell staining is often required to adequately image the samp Which of the following could NOT be seen clearly by the unaided eye? Select all that apply. -Answer-24um and 0.2um. The unaided eye can, on average, clearly resolve objects > 100 μm This type of microscope utilizes ultraviolet (UV) light to illuminate stained objects. - Answer-Fluorescence This type of microscope uses a specialized condenser and objective to amplify the slight differences between cells and background -Answer-Phase-Contrast his type of microscope enhances contrast between specimen and background but does not permit the visualization of intracellular structures -Answer-Dark Field This type of microscope uses neither halogen nor UV light sources but rather lasers to illuminate stained cells in high resolution -Answer-Confocal