Lab Quiz 1-- BIO 250L

Lab Quiz 1-- BIO 250L with correct answers well explained 100% guaranteed success what is the objective of air exposure? - correct answersto demonstrate that microorganism are around us define the medium? - correct answersliquid or gel designed to expose bacteria what are the two types if medium and what do they provide? - correct answersbroth and agar; they provide variable growth conditions in the form of growth and stability what three ways do you handle media? - correct answersmake sure that the top is on the bottom; plate is upside down make sure to seal the plate with parafilm store in correct temp for the bacteria define agar - correct answerschemical derived from seaweed that solidifies into a jelly-like substance and becomes a solid what are two types of agar? - correct answersplates and slants example of agar? - correct answersTSA--- trypticase soy agar what is the air exposure procedure? - correct answersopen the plate to expose it to the air for several minutes then seal with parafilm and place in an incubate at 22C for 24 hours then 4C until the next week what is the objective of a surface swab? - correct answersto demonstrate that their are microorganism and can only kill some what is sterilization and some examples? - correct answerswhen you kill all the bacteria; autoclave and radiation what is autoclave? - correct answersexample of sterilization when you use heat and pressure what is disinfection and an example? - correct answersonly kills some or most of the microorganisms; Lysol when streaking plates, be sure to work close to the Bunsen burner why?? - correct answersbecause hot air rises creating a convection current therefore all the bacteria floats up causing your work no to be contaminated cross hatch pattern is what and what is it used in? - correct answerssurface swab and zigzag long was and up and down what is the objective of aseptic technique and transferring culture - correct answersto create a subculture of Staphylococcus Aureus aseptic technique define and give some examples - correct answersany procedure you do to help with contamination wipe table, flame the loop, work close to Bunsen burner whats a subculture? - correct answerstransfer micro growth from one medium to another whats a pure culture? - correct answersculture of bacteria contaminating only one strain what medium do we work with in aseptic technique? - correct answersTSB- trypticase soy broth TSB is used over TSA why? - correct answersTSB is liquid and used to grow aerobic bacteria S. aureus is found where? - correct answersskin and respiratory tract S. aureus is a common cause of - correct answersfood poison, skin infection, respiratory disease, and staff what is the objective of a 4 way streak - correct answersto obtain a pure culture and dilute the bacteria what is the bacteria used in 4 way streak? - correct answersmicrococcus luteus what are the characteristics of m. luteus? - correct answersobligate aerobic, normal flora of the human skin, and isolated by flemming how do you label the media? - correct answersinitials, date, section, organism name, procedure why do you want to heat the loop until it is red hot? - correct answersyou want to kill any bacteria or microorganisms still on the loop