HISTOPATHOLOGY EXAM QUESTIONS
AND ANSWERS |VERIFIED
Routine Tissue Processing: CORRECT ANSWERS 1. Fixation
2. Decalcification
3. Dehydration
4. Clearing
5. Infiltration
6. Embedding
7. Trimming
8. Cutting
9. Staining
10. Mounting
11. Labeling
FIXATION CORRECT ANSWERS Two Mechanisms in Fixatioon CORRECT ANSWERS 1. Additive
2. Non-additive
Additive Fixatives: CORRECT ANSWERS 1. Formaldehyde
2. Mercury
3. Osmium tetroxide
Additives CORRECT ANSWERS - crosslinks or melecular complexes
Non-additives CORRECT ANSWERS - alters the tissue composition by removing bound H2O from H-bonds
Types of Fixatives accdng. to Composition: CORRECT ANSWERS 1. Simple
2. Compound
Simple Fixatives CORRECT ANSWERS 1. Aldehydes
2. Metallic fixative
3. Acids
4. Acetone
5. Alcohol
6. Heat
Metallic Fixatives CORRECT ANSWERS 1. Mercuric chloride
2. Chromate Fixatives
3. Lead fixatives
Chromate Fixatives CORRECT ANSWERS 1. Potassium dichromate 2. Chromic acid
3. Regard's (Muller's fluid)
4. Orth's fluid
Acid Fixatives CORRECT ANSWERS 1. Picric acid
2. Acetic acid
3. Osmic acid
Acetone CORRECT ANSWERS - a highly volatile simple fixative that preserves water diffusable enzymes
Alcohol CORRECT ANSWERS - a simple fixative used for smear preparation
Heat CORRECT ANSWERS - a non-chemical simple fixative which works by thermal coagulation of proteins
Picric Acid CORRECT ANSWERS - an acid fixative for glycogen demonstration
Acetic Acid CORRECT ANSWERS - an acid fixative used for preservation of nuclear components and destruction of cytoplasm
Osmic Acid CORRECT ANSWERS - acid fixative used for electron microscopy
37-40% CORRECT ANSWERS - the concentration of fomalin in stock solution
Types of Fixatives accdng to Action: CORRECT ANSWERS 1. Microanatomical fixatives
2. Cytological fixatives
3. Histochemical fixatives
Microanatomical Fixatives CORRECT ANSWERS 1. 10% Formol salines
2. 10% Neutral buffered formalin
3. Zenker's solution
4. Bouin's solution
Cytological Fixatives CORRECT ANSWERS 1. Nuclear
2. Cytoplasmic
Histochemical Fixatives CORRECT ANSWERS 1. 10% Formol saline
2. Absolute ethyl alchol
3. Acetone
4. Newcomer's fluid
10% Neutral Buffered Formalin CORRECT ANSWERS - a microanatomical fixative that
prevents black precipitate formation
AND ANSWERS |VERIFIED
Routine Tissue Processing: CORRECT ANSWERS 1. Fixation
2. Decalcification
3. Dehydration
4. Clearing
5. Infiltration
6. Embedding
7. Trimming
8. Cutting
9. Staining
10. Mounting
11. Labeling
FIXATION CORRECT ANSWERS Two Mechanisms in Fixatioon CORRECT ANSWERS 1. Additive
2. Non-additive
Additive Fixatives: CORRECT ANSWERS 1. Formaldehyde
2. Mercury
3. Osmium tetroxide
Additives CORRECT ANSWERS - crosslinks or melecular complexes
Non-additives CORRECT ANSWERS - alters the tissue composition by removing bound H2O from H-bonds
Types of Fixatives accdng. to Composition: CORRECT ANSWERS 1. Simple
2. Compound
Simple Fixatives CORRECT ANSWERS 1. Aldehydes
2. Metallic fixative
3. Acids
4. Acetone
5. Alcohol
6. Heat
Metallic Fixatives CORRECT ANSWERS 1. Mercuric chloride
2. Chromate Fixatives
3. Lead fixatives
Chromate Fixatives CORRECT ANSWERS 1. Potassium dichromate 2. Chromic acid
3. Regard's (Muller's fluid)
4. Orth's fluid
Acid Fixatives CORRECT ANSWERS 1. Picric acid
2. Acetic acid
3. Osmic acid
Acetone CORRECT ANSWERS - a highly volatile simple fixative that preserves water diffusable enzymes
Alcohol CORRECT ANSWERS - a simple fixative used for smear preparation
Heat CORRECT ANSWERS - a non-chemical simple fixative which works by thermal coagulation of proteins
Picric Acid CORRECT ANSWERS - an acid fixative for glycogen demonstration
Acetic Acid CORRECT ANSWERS - an acid fixative used for preservation of nuclear components and destruction of cytoplasm
Osmic Acid CORRECT ANSWERS - acid fixative used for electron microscopy
37-40% CORRECT ANSWERS - the concentration of fomalin in stock solution
Types of Fixatives accdng to Action: CORRECT ANSWERS 1. Microanatomical fixatives
2. Cytological fixatives
3. Histochemical fixatives
Microanatomical Fixatives CORRECT ANSWERS 1. 10% Formol salines
2. 10% Neutral buffered formalin
3. Zenker's solution
4. Bouin's solution
Cytological Fixatives CORRECT ANSWERS 1. Nuclear
2. Cytoplasmic
Histochemical Fixatives CORRECT ANSWERS 1. 10% Formol saline
2. Absolute ethyl alchol
3. Acetone
4. Newcomer's fluid
10% Neutral Buffered Formalin CORRECT ANSWERS - a microanatomical fixative that
prevents black precipitate formation
