Biotility BACE Practice Knowledge Exam 100% Pass

Biotility BACE Practice Knowledge Exam 100% Pass cell theory states All life forms are made from one or more cells. Cells only arise from pre-existing cells. The cell is the smallest form of life. E.coli is an example of a eukaryote false Quality Assurance ensure that all products meet standards of quality in manufacturing process reasearch and development (R AND D) identify candidate biopharmaceutical drugs Manufacturing make commercial quantities of biopharmaceutical drug Which structure regulates the flow of molecules into and out of the cell? Plasma Membrane Which bacterial cell component is commonly isolated and used to digest DNA? Restriction Enzyme polymerase chain reaction ( PCR) Heat-stable DNA polymerase Digesting DNA Restriction enzymes ( EcoRI) Running DNA gels Horizontal gel electrophoresis box Running protein gels ( PAGE) Vertical gel electrophoresis box Joining pieces of DNA DNA ligase Spectrophotometer Measuring protein or DNA concentration A technician reapeated the maltase reaction described above, but the water bath failed to reach 37°C ( it is warmed to 30°C). What is the most likely out come? The enzyme activity would decrease , yielding the same level of glucose over a longer time period. Reaguarding the out come in question 8 , select the most appropriate action for the technician. Report the problem to QA or individual in charge of repairs and place an out of service lable on the water bath The byproduct of carbon dioxide benefits the brewing and baking industries. carbon dioxide is produced by microorganisms ,usually yeast, that obtain their energy by: Anaerobic Respiration Where is the energy in the triphosphate (ATP) ? Phosphate bonds In a DNA double helix, the two DNA chains are held together by: Hydrogen bonds between the pair of bases How do the DNA molecules in various species differ from one to another? The number of genes and non-coding regions. The nucleotide strands of DNA are oriented in opposite directions in a structure that is described as : Anti-parallel primary (1°) structure Amino acids linked together by peptide bonds Secondary (2°) structure 3D polypeptide with alpha-helices and beta-sheets due to hydrogen bonding. Tertiary (3°) structure Final 3D protein shape Quaternary (4°) structure Multiple polypeptides forming a larger protein complex Correct order of assembly in the cell: amino acid, polypeptide, protein Why does increasing the temperature of the reactents increase the rate of chemical reaction? Heat energy causes the particales of the reactents to move faster, and therefore react with each other more frequently Why does the rate of chemical reaction increase with increasing temperature ? Increase temperature increase the frequency of collisions between molecules Why are enzyme solutions always prepare using a buffered solvent cell theory states All life forms are made from one or more cells. Cells only arise from pre-existing cells. The cell is the smallest form of life. E.coli is an example of a eukaryote false Quality Assurance ensure that all products meet standards of quality in manufacturing process reasearch and development (R AND D) identify candidate biopharmaceutical drugs Manufacturing make commercial quantities of biopharmaceutical drug Which structure regulates the flow of molecules into and out of the cell? Plasma Membrane Which bacterial cell component is commonly isolated and used to digest DNA? Restriction Enzyme polymerase chain reaction ( PCR) Heat-stable DNA polymerase Digesting DNA Restriction enzymes ( EcoRI) Running DNA gels Horizontal gel electrophoresis box Running protein gels ( PAGE) Vertical gel electrophoresis box Joining pieces of DNA DNA ligase Spectrophotometer Measuring protein or DNA concentration A technician reapeated the maltase reaction described above, but the water bath failed to reach 37°C ( it is warmed to 30°C). What is the most likely out come? The enzyme activity would decrease , yielding the same level of glucose over a longer time period. Reaguarding the out come in question 8 , select the most appropriate action for the technician. Report the problem to QA or individual in charge of repairs and place an out of service lable on the water bath The byproduct of carbon dioxide benefits the brewing and baking industries. carbon dioxide is produced by microorganisms ,usually yeast, that obtain their energy by: Anaerobic Respiration Where is the energy in the triphosphate (ATP) ? Phosphate bonds In a DNA double helix, the two DNA chains are held together by: Hydrogen bonds between the pair of bases How do the DNA molecules in various species differ from one to another? The number of genes and non-coding regions. The nucleotide strands of DNA are oriented in opposite directions in a structure that is described as : Anti-parallel primary (1°) structure Amino acids linked together by peptide bonds Secondary (2°) structure 3D polypeptide with alpha-helices and beta-sheets due to hydrogen bonding. Tertiary (3°) structure Final 3D protein shape Quaternary (4°) structure Multiple polypeptides forming a larger protein complex Correct order of assembly in the cell: amino acid, polypeptide, protein Why does increasing the temperature of the reactents increase the rate of chemical reaction? Heat energy causes the particales of the reactents to move faster, and therefore react with each other more frequently Why does the rate of chemical reaction increase with increasing temperature ? Increase temperature increase the frequency of collisions between molecules Why are enzyme solutions always prepare using a buffered solvent at a specific pH? A buffered solvent is a resistant to fluctuations in pH, because it is made with a combination of a weak acid and it's made with a combination of a weak acid and it's conjugate base. Enzymes lose function outside of a narrow pH range. Naturally occurring enzymes invovled with DNA replication, and which are also used as tools in biotechnology include : DNA polymerase , DNA ligase If a structural Gene's code is "TAC ATG CCC TTA ATC", What will the mRNA transcript be? AUG UAC GGG AAU UAG During the process of translation: mRNA is translated into proteins Where does translation take place in prokaryotes? At the ribosomes Humans have two copies of each cromosome, and therefore are: Diploid The observable traits of an organism are referred to as the: Phenotype The type of cell division in which haploid gametes are produced is called Meiosis What is the chance a child will be born with cystic fibrosis ( a homozygous recessive trait (aa)) if both the mom and dad are heterozygous (Aa) ? 25% chance of having the disorder If DNA segments changes from GGTTAG to GGATAG this is a: Nucleotide base substitution monocolonal atibodies for scientific research can be produced in human cells True How many grams is 2.6kg? 2600 Plasmid DNA is purified using an ion exchange column chromatography. why? Because DNA is negatively charged, binds to the positively charged resin, and is eluted with increasing salt.