Ascp MLT Exam Questions & Answers 2023 (A+ Graded 100% Correct).

Ascp MLT Exam Questions & Answers 2023 (A+ Graded 100% Correct). ACD/CPD/CPD2 - ANSWER 21 days CPDA-1 - ANSWER 35 days Infants HDFN from ABO - ANSWER Spherocytes 1st pregnancy O mother Permanent deferral - ANSWER Hepatitis after 11 HIV T. Cruzi CJD 1 unit or PRBC - ANSWER Raises hemoglobin 1g and hematocrit 3% Leukoreduced RBCs - ANSWER Less than 5 x 10^6 to prevent febrile non hemolytic reactions Cryoprecipitate - ANSWER Factor 8 vWF Fibrinogen For DIC 6 hours Platelets 1unit - ANSWER Irradiated blood - ANSWER Prevent GVHD Ulex europaeus - ANSWER Anti H lectin Dolichos biflorus - ANSWER Anti A1 lectin Amount of H greatest to least - ANSWER O A2 B A1 A1B Most immunogenicity antigen - ANSWER D Weak D - ANSWER Negative immediate spin and positive AHG Weak D donor - ANSWER Must be labeled Rh positive Weak D patient - ANSWER Receives D negative D control - ANSWER AB positive Any D negative IgM antibodies - ANSWER I H MN P1 Lewis IgG antibodies - ANSWER Rh group K Duffy Jk I, i antibody - ANSWER i converts to I as infant matures Destroyed by enzymes - ANSWER MN S Duffy Enhanced by enzymes - ANSWER Rh Kids Lewis I P1 Dosage - ANSWER MNS Rh Kids Duffy Top three acute/immediate transfusion reactions and mortality - ANSWER TRALI, hemolytic transfusion reactions and TACO IgE antibodies - ANSWER Mild Allergic reactions IgA antibodies - ANSWER Severe transfusion allergic reaction Anaphylactic Positive hemolysis with negative DAT - ANSWER Sickle cell crisis Thalassemia/G6PD deficient Unit overheated or frozen All cells hemolysis Kernicterus - ANSWER Excess bilirubin in newborn HDFN from Rh - ANSWER Retics DAT positive Immediate jaundice After first baby Rosette - ANSWER Screening for fetal hemorrhage Kleihauer Betke - ANSWER Quantitative fetal maternal bleed Primary immune response - ANSWER IgM Secondary immune response - ANSWER IgG Type 1 hypersensitivity - ANSWER Anaphylactic Immediate Type 2 hypersensitivity - ANSWER Antibody depending cytotoxicity Transfusion reactions Hashimotos Good pasture Type 3 hypersensitivity - ANSWER Immune complex Rheumatoid arthritis SLE Type 4 hyper sensitivity - ANSWER Delayed Monocytes and lymphocytes Ouchterlony - ANSWER Antibodies added to pre-cut wells in center of agar plate and patient Sera and standards are alternated in wells surrounding the center well EIA/ELISA - ANSWER Sandwich technique HCG Nephelometry - ANSWER Insoluble complexes Why is pass-through suspension scattered light absorbance is proportional to the number of insoluble complexes compared to standards Antibody concentration Immunofluorescence direct - ANSWER Add florescence labeled anti-body to patient tissue wash and examined under fluorescent microscope Immunofluorescence indirect - ANSWER Add patient serum to reagent wash add florescence label to anti-globulin wash and examined under microscope FPIA (Fluorescence Polarization Immunoassay) - ANSWER Add reagent antibody and fluorescent tact antigen to patient serum Increase polarize light as a negative test decrease polarized light as a positive test Sensitivity - ANSWER TP/ TP + FN x 100 Specificity - ANSWER TN / TN + FP x 100 Non lattice - ANSWER More sensitive immunoassays nephelometry Lattice - ANSWER Less sensitive C reactive protein - ANSWER Acute phase protein Inflammation Syphilis - ANSWER T palladium FTA abs TPI Dark field microscopy VDRL - ANSWER Syphilis CSF screening but can be positive for malaria RPR - ANSWER Charcoal for syphilis More sensitive but Les specific infectious mononucleosis - ANSWER EBV Lymphocytes B cells Burkets disease? Autoimmune diseases - ANSWER SLE .