BioChem C785 study guide PA with complete solution

BioChem C785 study guide PA with complete solution Complementary DNA and/or RNA molecules pair with each other by hydrogen bonding during replication, transcription, or translation. Which of the following pairs of molecules are complementary? (Select all that apply) Correct Answer- tRNA and mRNA mRNA and template DNA During DNA replication the parent strands ______ and DNA polymerase______. Correct Answer- separate, creates the new DNA strands The following sequence is one strand of the DNA that makes up the SNCA gene that codes for alpha- synuclein, a protein found in the brain. 5' ATGCGGTGACGG 3' Which of the following is the complementary second DNA strand? Correct Answer- 5' CCGTCACCGCAT 3' Assume you start with one copy of target DNA. How many copies of DNA will be made after 4 rounds of PCR (assume 100% reaction efficiency)? Correct Answer- 16 What are the necessary ingredients for a PCR reaction? Correct Answer- dNTPs; thermostable DNA polymerase; DNA primers; double-stranded DNA A frameshift mutation changed this sequence 5' AAC GCT TAG 3' to which of the following? Correct Answer- 3' GAT TCG CA 5' Which choice represents a silent mutation for this sequence: 5' GGU UGC ACU 3'? Correct Answer- 5' GGU UGC ACA 3' Beta thalassemia is a disease that causes low levels of hemoglobin. To inherit this autosomal disease, an individual must receive two mutant alleles. Which of the following likely represents the chromosome pairs of a healthy parent who has a child with beta thalassemia? (The yellow band represents the mutant allele and the green band represents the normal allele.) * Correct Answer- Option 3- The chromosomes of a healthy parent who has a child with beta thalassemia must be a carrier of the disease, i.e. the parent must have one copy of the (dominant) normal allele and one copy of the (recessive) mutant allele.