BIOS 251 Week 4 Lab; Microscopy

Learning Objectives: • Understand different microscopy techniques and theirlimitations • Identify various cell types and cellularstructures • Understand stainingtechniques • Understand coeliac disease and intestinalinflammation Introduction: Histology is an important tool of biology which helps us understand how tissues are structured and how they function. This is helpful when we are investigating diseased or malformed tissues. In this simulation you will examine a chicken intestine sample that is infected with a retrovirus and observe how the virus infects the lymphocytes (the cells that provide immune protection) and how it inhibits inflammation. Inflammation is a sign oftissue damage. You will use different microscopic techniques to examine chicken intestine slide. Try out the electron microscope Learn about fluorescence staining techniques Part 1: Complete Labster Microscopy Part 2: Report and Reflection Purpose: • Describe in your own words and in complete sentences, the purpose of this experiment. • The purpose of this experiment was to show the differences between the 3 microscopes we used and how to use each of them, while also learning the different kinds of magnifications and what we can see at each level. • Observations: List 3 observations you have made in this simulation. • Answer all the questions below: 1. Distinguish the light microscope from the electron microscope.(1point) 2. Draw the three types of muscle tissue (3points) 3. What type of cells lines the intestinal lumen?(1point) 4. Provide the function of the following cell junctions: (4points) a. Tight junctions: b. Adherens junctions: c. Gap junctions: d. Desmosomes: 5. Reflection: Reflect on at least 2 key concepts you have learned from this simulation. How would you relate it to the physiological functions of thebody?