Microbiology Lab Practicum Review_
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Key 1Terms
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& DefinitionsPage 1 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
How do you write a scientific name in proper form? - Capitalize genus
- all the rest- lowercase
- underline if writing (italicize or underline if typing)
Parts of Microscope: Stage platform that holds specimen slide
Parts of Microscope: Substage Condenser focuses light from the lamp onto the specimen
Parts of Microscope: Course Adjustment Knob knob used to make larger changes in focus (to by used on 4x and 10x
only)
10/20/2026, 4:13:18 AMPage 1 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
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Lab Practicum
LabReview_
Practicum
Key
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Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
,Microbiology Lab Practicum Review_
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Key 2Terms
of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
& DefinitionsPage 2 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
Parts of Microscope: Iris Diaphragm mechanism that controls the amount of light that reaches the specimen
Parts of Microscope: 4x Objective Lens Scanning Lens - 40x total magnification (with a 10x ocular lens
microscope)
Resolution the ability to discern the different aspects of a specimen from eachother
How do you calculate magnification? Objective Mag x Ocular Mag = Total Mag
How do you prepare a heat-fixed smear of proper - Aseptically obtain a sample of bacteria and place it on a clean slide
density for staining a broth culture? - Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix
10/20/2026, 4:13:18 AMPage 2 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
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Lab Practicum
LabReview_
Practicum
Key
Review_
Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
, Microbiology Lab Practicum Review_
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Key 3Terms
of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
& DefinitionsPage 3 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
How do you prepare a heat-fixed smear of proper - Place a drop of DI (deionized) water on a clean slide
density for staining a solid, slant culture? - Aseptically obtain a sample of bacteria on loop and mix into drop of
water
- Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix
What are the steps for preparing a Gram Staining - Heat-fixed emulsion slide
Procedure - 1 drop of crystal violet (primary stain) for 45 seconds
- Gently rinse with distilled water into Tupperware container
- Cover smear with Iodine stain (mordant) for 15 seconds
- Gently rinse slide with distilled water
- Trickle down slide with 95% ethanol (decolorizer) until run off is clear
- Gently rinse with distilled water
- 1 drop Safranin (counterstain) for 30 seconds
- Rinse with distilled water
- Gently blot dry in a tablet of bibulous paper
What color is a gram-positive reaction? purple/blue
10/20/2026, 4:13:18 AMPage 3 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
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Lab Practicum
LabReview_
Practicum
Key
Review_
Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
Page
Key 1Terms
of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
& DefinitionsPage 1 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
How do you write a scientific name in proper form? - Capitalize genus
- all the rest- lowercase
- underline if writing (italicize or underline if typing)
Parts of Microscope: Stage platform that holds specimen slide
Parts of Microscope: Substage Condenser focuses light from the lamp onto the specimen
Parts of Microscope: Course Adjustment Knob knob used to make larger changes in focus (to by used on 4x and 10x
only)
10/20/2026, 4:13:18 AMPage 1 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
Page 1 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
Page 1 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
Lab Practicum
LabReview_
Practicum
Key
Review_
Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
,Microbiology Lab Practicum Review_
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Key 2Terms
of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
& DefinitionsPage 2 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
Parts of Microscope: Iris Diaphragm mechanism that controls the amount of light that reaches the specimen
Parts of Microscope: 4x Objective Lens Scanning Lens - 40x total magnification (with a 10x ocular lens
microscope)
Resolution the ability to discern the different aspects of a specimen from eachother
How do you calculate magnification? Objective Mag x Ocular Mag = Total Mag
How do you prepare a heat-fixed smear of proper - Aseptically obtain a sample of bacteria and place it on a clean slide
density for staining a broth culture? - Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix
10/20/2026, 4:13:18 AMPage 2 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
Page 2 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
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Lab Practicum
LabReview_
Practicum
Key
Review_
Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
, Microbiology Lab Practicum Review_
Page
Key 3Terms
of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
& DefinitionsPage 3 of Page
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Lab Practicum Review_ Key
LabTerms
Practicum
Lab
& Definitions.pdf10/20/2026,
Practicum
Review_ Key
Review_
TermsKey
& Definitions.pdf10/20/2026,
Terms
4:09:33&AM
Definitions.pdf10/20/2026,
4:09:33 AM4:09:33 AM
How do you prepare a heat-fixed smear of proper - Place a drop of DI (deionized) water on a clean slide
density for staining a solid, slant culture? - Aseptically obtain a sample of bacteria on loop and mix into drop of
water
- Spread liquid over a small area of the slide (coin sized)
- Place slide on slide warmer (if air drying - set out at room temp)
- Once smear is completely dry, pass over flame 3 times to heat fix
What are the steps for preparing a Gram Staining - Heat-fixed emulsion slide
Procedure - 1 drop of crystal violet (primary stain) for 45 seconds
- Gently rinse with distilled water into Tupperware container
- Cover smear with Iodine stain (mordant) for 15 seconds
- Gently rinse slide with distilled water
- Trickle down slide with 95% ethanol (decolorizer) until run off is clear
- Gently rinse with distilled water
- 1 drop Safranin (counterstain) for 30 seconds
- Rinse with distilled water
- Gently blot dry in a tablet of bibulous paper
What color is a gram-positive reaction? purple/blue
10/20/2026, 4:13:18 AMPage 3 of 1910/20/2026https://www.stuvia.com/user/????????????????????"OP@)(@)(*#@(#*@#&*$^&)(*@)$*$(&(Y&*&%Y&*(@)&$(&%*&%*&(&%@&*($#(*&98oMicrobiology
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Lab Practicum
LabReview_
Practicum
Key
Review_
Terms &
Key
Definitions.pdf10/20/2026,
Terms Lab
& Definitions.pdf10/20/2026,
Practicum Review_
4:09:33Key
AMTerms
4:09:33&AM
Definitions.pdf10/20/2026, 4:09:33 AM
