MCB 3023 EXAM 2 QUESTIONS WITH VERIFIED ANSWERS
What are micro RNAs?
a. tRNAs
b. small regulatory RNAs
c. rRNAs
d. small mRNAs
e. mRNAs that encode for regulatory proteins - Answers - b. small regulatory RNAs
Replication of the bacterial chromosome begins at how many locations?
a. One
b. Three
c. Four
d. Two
e. Five - Answers - a. one
Which of these best differentiates DNA and RNA?
a. RNA contains ribose and DNA contains deoxyribose
b. RNA is single-stranded and DNA is double stranded
c. All statements are true
d. RNA contains the base uracil in place of thymine, which is in DNA - Answers - c. All
statements are true
Which enzyme is responsible for adding the final bases to the end of a chromosome
during DNA replication in eukaryal cells?
a. DNA polδ
b. DNA primase B
,c. topoisomerase
d. DNA polα
e. Telomerase - Answers - e. Telomerase
Term for cells that can naturally take up extracellular DNA
a. primed
b. susceptible
c. conjugant
d. porous
e. competent - Answers - e. competent
Suppose a mutant strain of Escherichia coli K12 contains a mutation in the trpE gene
and is no longer able to make tryptophan. What is this mutant called?
a. A phenotroph
b. An auxotroph
c. A lithotroph
d. A prototroph
e. An autotroph - Answers - b. An auxotroph
Which term applies to the transfer of genes from mother to daughter cell?
a. horizontal gene transfer
b. meiosis
c. transduction
d. vertical gene transfer
e. lateral gene transfer - Answers - d. vertical gene transfer
The process of molecular cloning was mainly brought about through the discovery of
these
,a. bacteriophages
b. restriction enzymes
c. transformations
d. conjugation events
e. transposons - Answers - b. restriction enzymes
Bacterial mutants have been very useful for studying and understanding:
a. capsule production
b. carbon metabolism
c. antibiotic resistance
d. all of these choices
e. biosynthetic pathways - Answers - d. all of these choices are correct
What is metagenomics?
a. A culture-dependent method that may be used to determine protein sequences
b. A culture -independent method that may be used for the isolation of different
organisms
c. A culture-dependent method that may be used to study groups of microbes in an
ecosystem
d. A culture-independent method that may be used to document microbial community
pools of genetic material
e. A culture-dependent method that may be used to find new enzymes - Answers - d. A
culture-independent method that may be used to document microbial community pools
of genetic material
The sequencing method which uses detection of pyrophosphate liberated when
nucleotides are added to a growing DNA chain is called:
a. Sanger dideoxy sequencing
b. Ion torrent sequencing
, c. Pyrosequencing
d. PacBio sequencing
e. Plus-minus sequencing - Answers - c. Pyrosequencing
Which of these is another term for the Sanger method of DNA sequencing?
a. chemical degradation method
b. restriction method
c. dideoxy sequencing
d. gel method
e. label method - Answers - c. dideoxy sequencing
Genes which confer invasiveness to a pathogen and whose GC content varies
significantly from that of the total genome constitute:
a. Symbiosis islands
b. Pathogenicity islands
c. SSU rRNA genes
d. Paralogs
e. Orthologs - Answers - b. Pathogenicity islands
Which of these would a signal peptide be used for in bacteria?
a. To regulate translation
b. To move a protein to the outside of the cell
c. To help a protein fold correctly
d. To help regulate the activity of an enzyme
e. To initiate DNA replication - Answers - b. To move a protein to the outside of the cell
Bacteriophages vary in their ability to infect different strains of bacteria. This is because
some bacteria contain _________ __________ that cut the phage DNA after it enters
the cell.
What are micro RNAs?
a. tRNAs
b. small regulatory RNAs
c. rRNAs
d. small mRNAs
e. mRNAs that encode for regulatory proteins - Answers - b. small regulatory RNAs
Replication of the bacterial chromosome begins at how many locations?
a. One
b. Three
c. Four
d. Two
e. Five - Answers - a. one
Which of these best differentiates DNA and RNA?
a. RNA contains ribose and DNA contains deoxyribose
b. RNA is single-stranded and DNA is double stranded
c. All statements are true
d. RNA contains the base uracil in place of thymine, which is in DNA - Answers - c. All
statements are true
Which enzyme is responsible for adding the final bases to the end of a chromosome
during DNA replication in eukaryal cells?
a. DNA polδ
b. DNA primase B
,c. topoisomerase
d. DNA polα
e. Telomerase - Answers - e. Telomerase
Term for cells that can naturally take up extracellular DNA
a. primed
b. susceptible
c. conjugant
d. porous
e. competent - Answers - e. competent
Suppose a mutant strain of Escherichia coli K12 contains a mutation in the trpE gene
and is no longer able to make tryptophan. What is this mutant called?
a. A phenotroph
b. An auxotroph
c. A lithotroph
d. A prototroph
e. An autotroph - Answers - b. An auxotroph
Which term applies to the transfer of genes from mother to daughter cell?
a. horizontal gene transfer
b. meiosis
c. transduction
d. vertical gene transfer
e. lateral gene transfer - Answers - d. vertical gene transfer
The process of molecular cloning was mainly brought about through the discovery of
these
,a. bacteriophages
b. restriction enzymes
c. transformations
d. conjugation events
e. transposons - Answers - b. restriction enzymes
Bacterial mutants have been very useful for studying and understanding:
a. capsule production
b. carbon metabolism
c. antibiotic resistance
d. all of these choices
e. biosynthetic pathways - Answers - d. all of these choices are correct
What is metagenomics?
a. A culture-dependent method that may be used to determine protein sequences
b. A culture -independent method that may be used for the isolation of different
organisms
c. A culture-dependent method that may be used to study groups of microbes in an
ecosystem
d. A culture-independent method that may be used to document microbial community
pools of genetic material
e. A culture-dependent method that may be used to find new enzymes - Answers - d. A
culture-independent method that may be used to document microbial community pools
of genetic material
The sequencing method which uses detection of pyrophosphate liberated when
nucleotides are added to a growing DNA chain is called:
a. Sanger dideoxy sequencing
b. Ion torrent sequencing
, c. Pyrosequencing
d. PacBio sequencing
e. Plus-minus sequencing - Answers - c. Pyrosequencing
Which of these is another term for the Sanger method of DNA sequencing?
a. chemical degradation method
b. restriction method
c. dideoxy sequencing
d. gel method
e. label method - Answers - c. dideoxy sequencing
Genes which confer invasiveness to a pathogen and whose GC content varies
significantly from that of the total genome constitute:
a. Symbiosis islands
b. Pathogenicity islands
c. SSU rRNA genes
d. Paralogs
e. Orthologs - Answers - b. Pathogenicity islands
Which of these would a signal peptide be used for in bacteria?
a. To regulate translation
b. To move a protein to the outside of the cell
c. To help a protein fold correctly
d. To help regulate the activity of an enzyme
e. To initiate DNA replication - Answers - b. To move a protein to the outside of the cell
Bacteriophages vary in their ability to infect different strains of bacteria. This is because
some bacteria contain _________ __________ that cut the phage DNA after it enters
the cell.
