BIO340 EXAM 3 (MOD 5 -6 ) QUESTIONS WITH
ACCURATE ANSWERS
During the denaturation step in PCR, what kind of bonds are broken so the two DNA
strands are separated? - ANSWER Hydrogen bonds
A lab is doing gel electrophoresis to determine the father of this child. Based on the
figure, who is the paternal parent of the child? - ANSWER Male 1 (MORE OF THE
DASHED LINES ACTUALLY MATCH UP)
The main goal of a PCR is to - ANSWER Amplify a fragment of DNA
Forward primers bind to the Template strand . - ANSWER Template strand
Professor Steven has asked you to amplify a gene, which of the following components
is NOT required for PCR? - ANSWER Ligase
What temperature do you need for the hot start? - ANSWER 95 degrees C
How many copies of DNA do you get after 4 full cycles? - ANSWER 16
In gel electrophoresis, DNA travels towards the negative electrode, because DNA is
positively charged. - ANSWER False
Forward primer sequence: 5'-ATGTTGACTC TAACTCGCAT CCGC-3'
Reverse primer sequence: 5'- ATGCCCAGA GGGACAAGCT GCCA -3'
Reverse primer region on coding strand:________________________ - ANSWER 5'-
TGGC AGCTTGTCCC TCTGGGCAT-3'
Find the proper annealing temperature for the forward and reverse primers from this
sequence using this website: - ANSWER Forward primer temperature: 68 degrees
Celsius.
Reverse primer temperature: 76 degrees Celsius.
, What is the correct order of enzyme action during DNA replication? Number the steps
from 1 to 7. - ANSWER 1-Binding to origin of replication (AT rich sequence)
2-Helicase/topoisomerase activity
3-Single-stranded binding proteins bind and prevent reannealing
4- Synthesis of RNA primers (priming)
5-DNA polymerization/proofreading
6-5'-3' exonuclease activity/DNA polymerization
7-Ligation
Use the following DNA strand in your responses to the next questions
3' TAC ATG ATC ATT TCA CGG AAT TTC TAG CAT GTA 5'
What is the mRNA strand produced from this DNA, starting with the start codon?
Hint: To find the start codon, you may need to read the DNA strand in both directions -
ANSWER 5' AUG CUA GAA AUU CCG UGA 3'
Which direction was the DNA strand transcribed in? - ANSWER Right to Left
What is the peptide sequence produce by this mRNA? Use the 3 letter amino acid
abbreviation found in the genetic code table below - ANSWER Leucine, Glu, Ile, Pro
If the DNA strand is transcribed from right to left and a nonsense mutation occurs in the
3rd codon of the mRNA strand, what would the polypeptide sequence be? - ANSWER
Leu, Stop
Which of the following 3 letter codon sequence serves as a stop codon? - ANSWER
UGA
Given the following DNA coding sequence: 5'-ACCTGTAGCG-3'. Which of the answers
below represents the mRNA sequence in the correct direction for this sequence? -
ANSWER RNA; 5'-ACCUGUAGCG-3'
Consider the DNA-template strand: 3' TGCTGGAGC 5'.What is the correct amino acid
sequence? - ANSWER Thr - Thr - Ser
The correct order of transcription and translation steps - ANSWER Initiation, elongation,
termination
Translation in prokaryotes can occur without transcription due to the lack of nucleus. -
ANSWER False
Recall the Central Dogma (DNA-mRNA-Protein). Which of the following would block
mature mRNA from being translated into protein? - ANSWER A substance that prevents
the transfer of amino acids to the growing polypeptide chain
ACCURATE ANSWERS
During the denaturation step in PCR, what kind of bonds are broken so the two DNA
strands are separated? - ANSWER Hydrogen bonds
A lab is doing gel electrophoresis to determine the father of this child. Based on the
figure, who is the paternal parent of the child? - ANSWER Male 1 (MORE OF THE
DASHED LINES ACTUALLY MATCH UP)
The main goal of a PCR is to - ANSWER Amplify a fragment of DNA
Forward primers bind to the Template strand . - ANSWER Template strand
Professor Steven has asked you to amplify a gene, which of the following components
is NOT required for PCR? - ANSWER Ligase
What temperature do you need for the hot start? - ANSWER 95 degrees C
How many copies of DNA do you get after 4 full cycles? - ANSWER 16
In gel electrophoresis, DNA travels towards the negative electrode, because DNA is
positively charged. - ANSWER False
Forward primer sequence: 5'-ATGTTGACTC TAACTCGCAT CCGC-3'
Reverse primer sequence: 5'- ATGCCCAGA GGGACAAGCT GCCA -3'
Reverse primer region on coding strand:________________________ - ANSWER 5'-
TGGC AGCTTGTCCC TCTGGGCAT-3'
Find the proper annealing temperature for the forward and reverse primers from this
sequence using this website: - ANSWER Forward primer temperature: 68 degrees
Celsius.
Reverse primer temperature: 76 degrees Celsius.
, What is the correct order of enzyme action during DNA replication? Number the steps
from 1 to 7. - ANSWER 1-Binding to origin of replication (AT rich sequence)
2-Helicase/topoisomerase activity
3-Single-stranded binding proteins bind and prevent reannealing
4- Synthesis of RNA primers (priming)
5-DNA polymerization/proofreading
6-5'-3' exonuclease activity/DNA polymerization
7-Ligation
Use the following DNA strand in your responses to the next questions
3' TAC ATG ATC ATT TCA CGG AAT TTC TAG CAT GTA 5'
What is the mRNA strand produced from this DNA, starting with the start codon?
Hint: To find the start codon, you may need to read the DNA strand in both directions -
ANSWER 5' AUG CUA GAA AUU CCG UGA 3'
Which direction was the DNA strand transcribed in? - ANSWER Right to Left
What is the peptide sequence produce by this mRNA? Use the 3 letter amino acid
abbreviation found in the genetic code table below - ANSWER Leucine, Glu, Ile, Pro
If the DNA strand is transcribed from right to left and a nonsense mutation occurs in the
3rd codon of the mRNA strand, what would the polypeptide sequence be? - ANSWER
Leu, Stop
Which of the following 3 letter codon sequence serves as a stop codon? - ANSWER
UGA
Given the following DNA coding sequence: 5'-ACCTGTAGCG-3'. Which of the answers
below represents the mRNA sequence in the correct direction for this sequence? -
ANSWER RNA; 5'-ACCUGUAGCG-3'
Consider the DNA-template strand: 3' TGCTGGAGC 5'.What is the correct amino acid
sequence? - ANSWER Thr - Thr - Ser
The correct order of transcription and translation steps - ANSWER Initiation, elongation,
termination
Translation in prokaryotes can occur without transcription due to the lack of nucleus. -
ANSWER False
Recall the Central Dogma (DNA-mRNA-Protein). Which of the following would block
mature mRNA from being translated into protein? - ANSWER A substance that prevents
the transfer of amino acids to the growing polypeptide chain
