MICRO 3060 LAB MID-TERM QUESTIONS WITH VERIFIED ANSWERS
Correct 48
Incorrect 00
1 of 48
Term
know the functional type, mechanism of action, what type of colonies
will grow, and how they can be distinguished on Blood Agar
Give this one a try later!
- purpose: detect carb. fermentation
- pos. reaction: yellow broth and gas bubble
- media used: glucose broth in durham tube for 24-48 hrs
- end: phenol red turns yellow (acidic) for pos. or red (neutral) for neg.
- functional type: general purpose, enriched, differential
- colony type: gram negative and gram positive
- distinguished: gamma (no break down), alpha (partial break down), beta (full
break down)
, - purpose: detect the formation of CO2 and ammonia
- pos. reaction: turns pink
- media used: urea agar slants with loose caps for O2
- end: phenol red turns pink (alkaline) for pos. test and stays yellow for neg. test
(neutral)
- purpose: detects if casein was hydrolyzed
- pos. reaction: clear zone
- media used: skim milk agar for 24 hrs
- end: clear zone for pos. test (bc casein ate away milk proteins) and white/cloudy
zone for neg. test
Don't know?
2 of 48
Term
define pure culture and CFU
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- pure culture: population of cells arising from a single cell
- CFU (colony forming units): units of measurement for a pour plate
- halophile: microbes that require the presence of salt in their environment
- halotolerant: ability of organisms to grow at salt concentrations higher than those
required for growth but can also grow without
- acidophile: 1-5.5 pH
- neutrophile: 5.5-8.5 pH
, - alkaliphile: 7.5-11 pH
a liquid or gelatinous substance containing nutrients in which microorganisms, cells,
or tissues are grown on
Don't know?
3 of 48
Definition
- purpose: measures the use of citrate as a carbon source
- pos. reaction: turns blue
- media used: citrate agar slant (streak/stab) for 24-48 hrs
- end: bromothylmol blue turns blue (alkaline) for pos. test and green
(neutral) for neg. test
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know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: H2S Production
know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: Casein Hydrolysis
, know the purpose, what a positive reaction would look like, what media and/
or reagents were used, and how the media and/or reagents work to show
negative and positive reactions Citrate Agar Test
know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: Urease Test
Don't know?
4 of 48
Term
describe endospores, their function, and know which genera produce
them
Give this one a try later!
- agar plate: shape, margin, elevation, size, texture, color, opacity
- agar slant: shape (filiform, absorbent, beaded, diffuse, rhizoid, echinulate)
- nutrient broth: location of growth (clear, turbid, flocculent, pellicle, sediment)
- a bacterial structures that allow the cell to survive in harsh conditions
- function: preserves genetic material in harsh times by becoming dormant
- genera: bacillus and clostridium
- a protective outer structure that correlates to the virulence of the bacteria
regulating the disease causing ability
Correct 48
Incorrect 00
1 of 48
Term
know the functional type, mechanism of action, what type of colonies
will grow, and how they can be distinguished on Blood Agar
Give this one a try later!
- purpose: detect carb. fermentation
- pos. reaction: yellow broth and gas bubble
- media used: glucose broth in durham tube for 24-48 hrs
- end: phenol red turns yellow (acidic) for pos. or red (neutral) for neg.
- functional type: general purpose, enriched, differential
- colony type: gram negative and gram positive
- distinguished: gamma (no break down), alpha (partial break down), beta (full
break down)
, - purpose: detect the formation of CO2 and ammonia
- pos. reaction: turns pink
- media used: urea agar slants with loose caps for O2
- end: phenol red turns pink (alkaline) for pos. test and stays yellow for neg. test
(neutral)
- purpose: detects if casein was hydrolyzed
- pos. reaction: clear zone
- media used: skim milk agar for 24 hrs
- end: clear zone for pos. test (bc casein ate away milk proteins) and white/cloudy
zone for neg. test
Don't know?
2 of 48
Term
define pure culture and CFU
Give this one a try later!
- pure culture: population of cells arising from a single cell
- CFU (colony forming units): units of measurement for a pour plate
- halophile: microbes that require the presence of salt in their environment
- halotolerant: ability of organisms to grow at salt concentrations higher than those
required for growth but can also grow without
- acidophile: 1-5.5 pH
- neutrophile: 5.5-8.5 pH
, - alkaliphile: 7.5-11 pH
a liquid or gelatinous substance containing nutrients in which microorganisms, cells,
or tissues are grown on
Don't know?
3 of 48
Definition
- purpose: measures the use of citrate as a carbon source
- pos. reaction: turns blue
- media used: citrate agar slant (streak/stab) for 24-48 hrs
- end: bromothylmol blue turns blue (alkaline) for pos. test and green
(neutral) for neg. test
Give this one a try later!
know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: H2S Production
know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: Casein Hydrolysis
, know the purpose, what a positive reaction would look like, what media and/
or reagents were used, and how the media and/or reagents work to show
negative and positive reactions Citrate Agar Test
know the purpose, what a positive reaction would look like, what media and/ or
reagents were used, and how the media and/or reagents work to show negative
and positive reactions: Urease Test
Don't know?
4 of 48
Term
describe endospores, their function, and know which genera produce
them
Give this one a try later!
- agar plate: shape, margin, elevation, size, texture, color, opacity
- agar slant: shape (filiform, absorbent, beaded, diffuse, rhizoid, echinulate)
- nutrient broth: location of growth (clear, turbid, flocculent, pellicle, sediment)
- a bacterial structures that allow the cell to survive in harsh conditions
- function: preserves genetic material in harsh times by becoming dormant
- genera: bacillus and clostridium
- a protective outer structure that correlates to the virulence of the bacteria
regulating the disease causing ability
