MICR 3060 FINAL EXAM QUESTIONS WITH VERIFIED ANSWERS
Correct 25
Incorrect 00
1 of 25
Term
Where is Staph usually found on humans?
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Nasal passage, skin, throat A plate that has 30 - 300 colonies.
Growth, resistance to amp, no lawn of
growth, isolated colonies, and Saliva, tears, mucus, and egg whites
fluorescence
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2 of 25
,Term
Describe the method used to heat shock and transform E. Coli with
pGLO.
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It typically exists on the skin and competes with other microorganisms. It can cause
infection if the skin is opened.
Reagents and inoculation tubes were kept on ice. When ready, both tubes
were placed in a hot water bath for 50 seconds and then removed.
Heat shock will increase the permeability of the cell membrane.
1. Centrifuge
2. Add SDS (detergent) to break down the cell wall and membrane.
3. Add Proteinase K and heat to denature proteins in the cell.
4. Precipitate the DNA from the solution with ethanol or isopropyl alcohol.
A piece of velveteen was placed on a replica plating block. The master plate was
pressed onto the block.
The nutrient plate was then pressed onto the block at the same position as the
master plate (denoted by mark).
The streptomycin plate was then pressed onto the block.
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3 of 25
, Term
What would the predictions for -pGLO on LB/amp
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No growth, no resistance to amp, no lawn of growth, no isolated colonies, and
no fluorescence
GFP - green fluorescent protein
AMP - ampicillin
BLA - beta lactamase gene
ARA - arabinose
Bacteria that have taken up pGLO will display the bla gene, which makes them
resistant to ampicillin. They will be able to grow on plates treated with ampicillin.
GFP will fluoresce green when exposed to UV light. This mechanism is under the
control of arabinose.
Growth, resistance to amp, a lawn of growth, isolated colonies, and fluorescence
It will break the beta 1,4 glycosidic bonds between NAG and NAM in
peptidoglycan.
Don't know?
4 of 25
Term
Correct 25
Incorrect 00
1 of 25
Term
Where is Staph usually found on humans?
Give this one a try later!
Nasal passage, skin, throat A plate that has 30 - 300 colonies.
Growth, resistance to amp, no lawn of
growth, isolated colonies, and Saliva, tears, mucus, and egg whites
fluorescence
Don't know?
2 of 25
,Term
Describe the method used to heat shock and transform E. Coli with
pGLO.
Give this one a try later!
It typically exists on the skin and competes with other microorganisms. It can cause
infection if the skin is opened.
Reagents and inoculation tubes were kept on ice. When ready, both tubes
were placed in a hot water bath for 50 seconds and then removed.
Heat shock will increase the permeability of the cell membrane.
1. Centrifuge
2. Add SDS (detergent) to break down the cell wall and membrane.
3. Add Proteinase K and heat to denature proteins in the cell.
4. Precipitate the DNA from the solution with ethanol or isopropyl alcohol.
A piece of velveteen was placed on a replica plating block. The master plate was
pressed onto the block.
The nutrient plate was then pressed onto the block at the same position as the
master plate (denoted by mark).
The streptomycin plate was then pressed onto the block.
Don't know?
3 of 25
, Term
What would the predictions for -pGLO on LB/amp
Give this one a try later!
No growth, no resistance to amp, no lawn of growth, no isolated colonies, and
no fluorescence
GFP - green fluorescent protein
AMP - ampicillin
BLA - beta lactamase gene
ARA - arabinose
Bacteria that have taken up pGLO will display the bla gene, which makes them
resistant to ampicillin. They will be able to grow on plates treated with ampicillin.
GFP will fluoresce green when exposed to UV light. This mechanism is under the
control of arabinose.
Growth, resistance to amp, a lawn of growth, isolated colonies, and fluorescence
It will break the beta 1,4 glycosidic bonds between NAG and NAM in
peptidoglycan.
Don't know?
4 of 25
Term
