BIOL 134 (5) Questions with CORRECT Answers
Question:
Conversions kilo-10^3(1000)
Answer:
Milli-10^-3(.001) Micro(0.000001) Nano(0.000000001) G to kg- 1g to 0.001 kg m to
cm: 1m to 100cm m to mm: 1m to 1000mm L to ml: 1L to 1000ml
Question:
Why are Proteinase K and Rnase used in the isolation of DNA
Answer:
Proteinase K - is an enzyme used to digest proteins Rnaseprotein that cuts RNA
Question:
How is genomic DNA removed from solution during the isolation of plasmid DNA?
Answer:
Centrifuge the plasmid DNA and keep the liquid then add Naacetate and ethanol and
will give you your purified DNA
Question:
What are the properties of restriction enzymes?
Answer:
cut and cleaves DNA Molecules,targets a specific sequences
Question:
Why are restriction enzymes useful in gene cloning?
Answer:
you are able to get the specific part of the DNA that you want to clone
Question:
What are the properties of plasmids used for gene cloning?
Answer:
circular DNA molecules, multiple cloning sites, small, origin of replication, can
have a resistance gene
Question:
Conversions kilo-10^3(1000)
Answer:
Milli-10^-3(.001) Micro(0.000001) Nano(0.000000001) G to kg- 1g to 0.001 kg m to
cm: 1m to 100cm m to mm: 1m to 1000mm L to ml: 1L to 1000ml
Question:
Why are Proteinase K and Rnase used in the isolation of DNA
Answer:
Proteinase K - is an enzyme used to digest proteins Rnaseprotein that cuts RNA
Question:
How is genomic DNA removed from solution during the isolation of plasmid DNA?
Answer:
Centrifuge the plasmid DNA and keep the liquid then add Naacetate and ethanol and
will give you your purified DNA
Question:
What are the properties of restriction enzymes?
Answer:
cut and cleaves DNA Molecules,targets a specific sequences
Question:
Why are restriction enzymes useful in gene cloning?
Answer:
you are able to get the specific part of the DNA that you want to clone
Question:
What are the properties of plasmids used for gene cloning?
Answer:
circular DNA molecules, multiple cloning sites, small, origin of replication, can
have a resistance gene
