GENETICS: A CONCEPTUAL APPROACH FINAL
PAPER SOLVED QUESTIONS WITH COMPLETE
ANSWERS
◉ DNA POL delta.
Answer: elongates the laggin strands, EUK
◉ DNA Cloning LAC Z 3 products 1) No Vector, 2) Vector - no insert,
3) Vector + Insert.
Answer: 1) No Vector - not amp resistant
2) Vector - no insert amp resistant, lacz intact
3) Vector + Insert - no lacz
White = carrying recombinant plasmid
◉ 3 steps in PCR.
Answer: 1) Denaturation 95 C
2) Annealing 55-65 C
3) Synthesis 72 C
◉ Sanger Method
for DNA Sequencing.
, Answer: use dideoxynucleotides (ddNTP) create premature stop and
make the one that floresses
◉ Shotgun Sequencing.
Answer: sequence random fragments at a time
adapters on sequences bind to beads in wells
◉ Forward Genetics.
Answer: Induce mutations ➣ Find mutant in process of interest ➣
Mutant analysis➣Map gene ➣Sequence mutant allele ➣Study
biochemistry of protein
◉ Reverse Genetics.
Answer: Start with gene sequence ➣Induce mutation ➣Study
mutant phenotype/Study biochemistry of protein
◉ RNAi.
Answer: Interference by injection of complementary rna into genes,
silences the gene
◉ CRISPR/ Cas9.
Answer: Clustered Regularly-Interspaced Short Palindromic Repeats
Makes ds breaks at specific places (potential for gene editing)
PAPER SOLVED QUESTIONS WITH COMPLETE
ANSWERS
◉ DNA POL delta.
Answer: elongates the laggin strands, EUK
◉ DNA Cloning LAC Z 3 products 1) No Vector, 2) Vector - no insert,
3) Vector + Insert.
Answer: 1) No Vector - not amp resistant
2) Vector - no insert amp resistant, lacz intact
3) Vector + Insert - no lacz
White = carrying recombinant plasmid
◉ 3 steps in PCR.
Answer: 1) Denaturation 95 C
2) Annealing 55-65 C
3) Synthesis 72 C
◉ Sanger Method
for DNA Sequencing.
, Answer: use dideoxynucleotides (ddNTP) create premature stop and
make the one that floresses
◉ Shotgun Sequencing.
Answer: sequence random fragments at a time
adapters on sequences bind to beads in wells
◉ Forward Genetics.
Answer: Induce mutations ➣ Find mutant in process of interest ➣
Mutant analysis➣Map gene ➣Sequence mutant allele ➣Study
biochemistry of protein
◉ Reverse Genetics.
Answer: Start with gene sequence ➣Induce mutation ➣Study
mutant phenotype/Study biochemistry of protein
◉ RNAi.
Answer: Interference by injection of complementary rna into genes,
silences the gene
◉ CRISPR/ Cas9.
Answer: Clustered Regularly-Interspaced Short Palindromic Repeats
Makes ds breaks at specific places (potential for gene editing)
