SLLE BLOOD BANK UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS
Question:
Rh & ABO chromosomes
Answer:
Rh - chromosome 1 ABO - chromosome 9
Question:
A Antigen terminal sugar
Answer:
N-acetylgalactosamine
Question:
B antigen terminal sugar
Answer:
D-galactose
Question:
H antigen terminal sugar
Answer:
L-fucose
Question:
Blood groups with greatest amount of H antigen to least amount
Answer:
O > A2 > B > A2B > A1 > A1B
Question:
Differentiating A1 from A2
Answer:
Lectin
Question:
Individual with A2 blood type, which blood group should be transfused?
Answer:
O or A2 because individuals with A2 blood have an antibody against A1
Question:
No H antigen
Answer:
Bombay (hh) genotype
Question:
ABO antibodies
Answer:
IgM (Group A and Group
B) IgG (Group O)
, Question:
Detection of antigen-antibody reactions in blood bank by two forms
Answer:
1. Hemolysis (activation of complement)
2. Agglutination (clumping)
Question:
Group I Discrepancies
Answer:
Missing or weak Antibodies
Due to Newborns, old
age, lymphoma, BM transplant, or immunosuppressive drugs
Solution: Incubate at room temp for 15 minutes
Question:
Group II Discrepancies
Answer:
Missing or weak Antigens
Due to subgroups of A & B Leukemia, Hodgkin's disease
Solution: Wash cells with normal saline
Question:
Group III Discrepancies
Answer:
Plasma or protein abnormalities
Due to: Multiple Myeloma, Hodgkin's Lymphoma or Roleaux formation
Solution: Wash cells with saline or few drops, if agglutination is still there then
its true agglutination and clumping will remain
Question:
Group IV Discrepancies
Answer:
Miscellaneous problems
Could be due to viral or
bacterial infection stem cell transplantation
Question:
HLA chromosome
Answer:
Chromosome 6
Question:
MHC I
Answer:
a transmembrane glycoprotein, found on all nucleated cells
Question:
MHC II
Answer:
Found on anti-gen presenting cells (macrophages, B cells, and activated T cells;
signal helper T cells, dendritic cells, neutrophils)
CORRECT ANSWERS
Question:
Rh & ABO chromosomes
Answer:
Rh - chromosome 1 ABO - chromosome 9
Question:
A Antigen terminal sugar
Answer:
N-acetylgalactosamine
Question:
B antigen terminal sugar
Answer:
D-galactose
Question:
H antigen terminal sugar
Answer:
L-fucose
Question:
Blood groups with greatest amount of H antigen to least amount
Answer:
O > A2 > B > A2B > A1 > A1B
Question:
Differentiating A1 from A2
Answer:
Lectin
Question:
Individual with A2 blood type, which blood group should be transfused?
Answer:
O or A2 because individuals with A2 blood have an antibody against A1
Question:
No H antigen
Answer:
Bombay (hh) genotype
Question:
ABO antibodies
Answer:
IgM (Group A and Group
B) IgG (Group O)
, Question:
Detection of antigen-antibody reactions in blood bank by two forms
Answer:
1. Hemolysis (activation of complement)
2. Agglutination (clumping)
Question:
Group I Discrepancies
Answer:
Missing or weak Antibodies
Due to Newborns, old
age, lymphoma, BM transplant, or immunosuppressive drugs
Solution: Incubate at room temp for 15 minutes
Question:
Group II Discrepancies
Answer:
Missing or weak Antigens
Due to subgroups of A & B Leukemia, Hodgkin's disease
Solution: Wash cells with normal saline
Question:
Group III Discrepancies
Answer:
Plasma or protein abnormalities
Due to: Multiple Myeloma, Hodgkin's Lymphoma or Roleaux formation
Solution: Wash cells with saline or few drops, if agglutination is still there then
its true agglutination and clumping will remain
Question:
Group IV Discrepancies
Answer:
Miscellaneous problems
Could be due to viral or
bacterial infection stem cell transplantation
Question:
HLA chromosome
Answer:
Chromosome 6
Question:
MHC I
Answer:
a transmembrane glycoprotein, found on all nucleated cells
Question:
MHC II
Answer:
Found on anti-gen presenting cells (macrophages, B cells, and activated T cells;
signal helper T cells, dendritic cells, neutrophils)
