HT ASCP EXAM UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS
Question:
Alcian Blue
Answer:
The demonstration of acid mucopolysaccharides
Question:
Alcian Blue-PAS
Answer:
To differentiate b/w neutral and acidic mucosubstances
detection of intestinal metaplasia
Question:
Alcian Yellow
Answer:
Alcian yellow is used in histology in conjunction with toluidine blue to help
visualize
H. pylori
The resulting structures are stained as follows - Mucin: yellow - H. pylori: blue
- Other cellular components/background: shades of blue
Question:
Bielschowsky
Answer:
Demonstrate nerve fivers and the presence of neurofibrillary tangles and
senile plaques in Alzheimer disease
Question:
Carbol‐fuchsin (Kinyoun, Ziehl‐Neelsen, Fite, auramine‐rhodamine)
Answer:
to detect the presence of acid-fast mycobacteria in tissue sections
Question:
Colloidal Iron
Answer:
Identification of carboxylated and sulfated mucopolysaccharides and
glycoprotein mucin in tissue
Question:
Congo Red
Answer:
Demonstration of amyloid in tissues
Question:
Crystal Violet
Answer:
Rapid screening method for amyloid but is not as specific as Congo red
, Question:
Fontana‐Masson
Answer:
The demonstration of argentaffin substances such as melanin, argentaffin
granules of carcinoid tumors, and some neurosecretory granules
Neuroendocrine cells, neuroendocrine tumors, and melanoma
Question:
Giemsa
Answer:
to permit differentiation of cells present in hematopoietic tissue.
also used for the demonstration of some microorganisms
Question:
Gram
Answer:
identification of Gram-positive and Gram-negative microorganisms in tissue
samples
Question:
Grocott/Gomori methenamine‐silver (GMS)
Answer:
demonstration of urates in tissue.
A disturbance of uric-acid metabolism known as gout can cause the deposition
of urate crystals, especially around joints and in soft tissues.
Collection of the urate crystals are known as gouty tophi
Question:
Melanin bleach
Answer:
Bleaching with potassium permanganate or hydrogen peroxide is used to
remove melanin to examine cellular morphology
Question:
Hematoxylin & Eosin (H&E)
Answer:
Stain nucleus blue/purple
Extracellular matrix/cytoplasm pink
Question:
Luxol fast blue (LFB/cresyl echt violet)
Answer:
Demonstration of myelin in tissue sections.
When an axon degenerates, the myelin covering breaks down into simpler
lipids that will be removed eventually
Question:
Movat pentachrome
Answer:
to highlight the various constituents of connective tissue, especially
cardiovascular tissue, by five colors in a single stained slide
CORRECT ANSWERS
Question:
Alcian Blue
Answer:
The demonstration of acid mucopolysaccharides
Question:
Alcian Blue-PAS
Answer:
To differentiate b/w neutral and acidic mucosubstances
detection of intestinal metaplasia
Question:
Alcian Yellow
Answer:
Alcian yellow is used in histology in conjunction with toluidine blue to help
visualize
H. pylori
The resulting structures are stained as follows - Mucin: yellow - H. pylori: blue
- Other cellular components/background: shades of blue
Question:
Bielschowsky
Answer:
Demonstrate nerve fivers and the presence of neurofibrillary tangles and
senile plaques in Alzheimer disease
Question:
Carbol‐fuchsin (Kinyoun, Ziehl‐Neelsen, Fite, auramine‐rhodamine)
Answer:
to detect the presence of acid-fast mycobacteria in tissue sections
Question:
Colloidal Iron
Answer:
Identification of carboxylated and sulfated mucopolysaccharides and
glycoprotein mucin in tissue
Question:
Congo Red
Answer:
Demonstration of amyloid in tissues
Question:
Crystal Violet
Answer:
Rapid screening method for amyloid but is not as specific as Congo red
, Question:
Fontana‐Masson
Answer:
The demonstration of argentaffin substances such as melanin, argentaffin
granules of carcinoid tumors, and some neurosecretory granules
Neuroendocrine cells, neuroendocrine tumors, and melanoma
Question:
Giemsa
Answer:
to permit differentiation of cells present in hematopoietic tissue.
also used for the demonstration of some microorganisms
Question:
Gram
Answer:
identification of Gram-positive and Gram-negative microorganisms in tissue
samples
Question:
Grocott/Gomori methenamine‐silver (GMS)
Answer:
demonstration of urates in tissue.
A disturbance of uric-acid metabolism known as gout can cause the deposition
of urate crystals, especially around joints and in soft tissues.
Collection of the urate crystals are known as gouty tophi
Question:
Melanin bleach
Answer:
Bleaching with potassium permanganate or hydrogen peroxide is used to
remove melanin to examine cellular morphology
Question:
Hematoxylin & Eosin (H&E)
Answer:
Stain nucleus blue/purple
Extracellular matrix/cytoplasm pink
Question:
Luxol fast blue (LFB/cresyl echt violet)
Answer:
Demonstration of myelin in tissue sections.
When an axon degenerates, the myelin covering breaks down into simpler
lipids that will be removed eventually
Question:
Movat pentachrome
Answer:
to highlight the various constituents of connective tissue, especially
cardiovascular tissue, by five colors in a single stained slide
