MICROBIOLOGY 2117 ACTUAL EXAMS SCRIPT
QUESTIONS AND ANSWERS SURE A+
✔✔generation time - ✔✔the time required for a bacteria cell to grown and divide
✔✔generation time of tuberculosis - ✔✔15-20 hrs
✔✔generation time of E coli - ✔✔20 minutes or less
✔✔why generation time of tb and ecoli significant - ✔✔growth and spread of tb is harder
but ecoli spreads faster
✔✔growth curve of bacteria - ✔✔
✔✔lag phase - ✔✔adjusting to the new environment
getting used to the new enzymes and nutrients
✔✔log or exponential phase - ✔✔metabolic rate increase
rapid chromosome replication
growth & reproduction
✔✔stationary phase - ✔✔nutrients decrease
waste accumulate
, metabolism rate goes down
dying cells = number of cells being produced
✔✔death phase - ✔✔nutrients are not added
waste are not removed
cells die faster than they are produced
✔✔methods of counting bacteria - ✔✔dilution count
turbidity
✔✔dilution count - ✔✔dilute the medium and count the number of colonies
✔✔turbidity - ✔✔cloudiness
measured by spectrophotometer
✔✔if you have 2 tubes of bacteria and one is more turbid than another, what does that
mean - ✔✔more turbid means greater bacterial growth
✔✔metabolism - ✔✔all the chemical reactions in the body, anabolic and catabolic
✔✔catabolism - ✔✔breakdown of bonds
✔✔anabolism - ✔✔syntheses reactions in an organism
✔✔making proteins from amino acids is - ✔✔anabolic reaction
✔✔which reactions tend to release energy - ✔✔carabolic
✔✔is what form is this energy - ✔✔atp
✔✔most enzymes are what type of organic compounds - ✔✔proteins
✔✔where in the cell are they made - ✔✔ribosomes
✔✔how does the cell know which enzyme to make - ✔✔dna code the enzymes
information to mrna
✔✔biological catalyst - ✔✔chemicals that increase the likelihood of a reaction
✔✔what does it meant to say that an enzyme lowers the activation energy of a reaction
- ✔✔inhibitors can be permanent or reversible
✔✔enzymes are substrate specific - ✔✔substrates bind to its specific active site
QUESTIONS AND ANSWERS SURE A+
✔✔generation time - ✔✔the time required for a bacteria cell to grown and divide
✔✔generation time of tuberculosis - ✔✔15-20 hrs
✔✔generation time of E coli - ✔✔20 minutes or less
✔✔why generation time of tb and ecoli significant - ✔✔growth and spread of tb is harder
but ecoli spreads faster
✔✔growth curve of bacteria - ✔✔
✔✔lag phase - ✔✔adjusting to the new environment
getting used to the new enzymes and nutrients
✔✔log or exponential phase - ✔✔metabolic rate increase
rapid chromosome replication
growth & reproduction
✔✔stationary phase - ✔✔nutrients decrease
waste accumulate
, metabolism rate goes down
dying cells = number of cells being produced
✔✔death phase - ✔✔nutrients are not added
waste are not removed
cells die faster than they are produced
✔✔methods of counting bacteria - ✔✔dilution count
turbidity
✔✔dilution count - ✔✔dilute the medium and count the number of colonies
✔✔turbidity - ✔✔cloudiness
measured by spectrophotometer
✔✔if you have 2 tubes of bacteria and one is more turbid than another, what does that
mean - ✔✔more turbid means greater bacterial growth
✔✔metabolism - ✔✔all the chemical reactions in the body, anabolic and catabolic
✔✔catabolism - ✔✔breakdown of bonds
✔✔anabolism - ✔✔syntheses reactions in an organism
✔✔making proteins from amino acids is - ✔✔anabolic reaction
✔✔which reactions tend to release energy - ✔✔carabolic
✔✔is what form is this energy - ✔✔atp
✔✔most enzymes are what type of organic compounds - ✔✔proteins
✔✔where in the cell are they made - ✔✔ribosomes
✔✔how does the cell know which enzyme to make - ✔✔dna code the enzymes
information to mrna
✔✔biological catalyst - ✔✔chemicals that increase the likelihood of a reaction
✔✔what does it meant to say that an enzyme lowers the activation energy of a reaction
- ✔✔inhibitors can be permanent or reversible
✔✔enzymes are substrate specific - ✔✔substrates bind to its specific active site
