TSI COLLEGE READINESS ASSESSMENT 2026 READING WRITING AND MATH COMPLETE STUDY GUIDE AND SOLUTIONS

TSI COLLEGE READINESS ASSESSMENT
2026 READING WRITING AND MATH
COMPLETE STUDY GUIDE AND SOLUTIONS


◉ What is a TSI (Triple Sugar Iron) Test also referred to as?
Answer: Kligers Iron Test


◉ What does a TSI Test, test for?
Answer: Bacteria's ability to:
-Ferment glucose and lactose/sucrose
-Produce gas
-Reduce Sulfur


◉ What are the ingredients of this media?
Answer: Glucose, Sucrose, and Lactose


◉ What is the ratio of Glucose, Sucrose, and Lactose in this media?
Answer: 1:10:10


◉ What is the sulfur source in a TSI Test?

, Answer: Sodium Thiosulfate


◉ What is the fermentation indicator in a TSI Test?
Answer: Phenol Red


◉ What is the hydrogen sulfide indicator in a TSI Test?
Answer: Ferrous Sulfate


◉ What are the 2 steps to inoculating this media?
Answer: 1.) Stab the butt
2.) Fishtail the slant


◉ In the Fermentation of sugars portion of a TSI test, which sugar is
limited, and why?
Answer: Glucose, because it is bacteria's primary source of carbon


◉ Which sugar will bacteria always try to ferment first?
Answer: Glucose


◉ If ONLY glucose is fermented, what color will the slant and butt
be?
Answer: Red slant, Yellow butt