MICRO 020 LAB Actual EXAM Questions And
CORRECT Answers
Aseptic technique includes...
(a) sterilization of transfer tools before and after use
(b) disinfection of bench tops
(c) washing hands
(d) all of the above - CORRECT ANSWERS (d) all of the above
Placing the cap of a broth cultures up on the bench top is acceptable
True/False? - CORRECT ANSWERS False
Why is it important to allow the inoculating loop to cool prior to obtaining the inoculum? - CORRECT
ANSWERS to avoid frying the cell
A pure culture can be prepared from a mixed broth or a mixed agar slant culture directly
True/False - CORRECT ANSWERS False
Which of the following is a benefit of using the streak plate method for obtaining isolated colonies
compared to the pour plate method?
(a) the streak plate method allows you to observe colonies on the surface and deep in the agar
(b) the pour plate method does not require serial dilutions
(c) the streak plate method requires serial dilutions
(d) the streak plate method does not require serial dilutions - CORRECT ANSWERS (d) the
streak plate method does not require serial dilutions
When preparing a streak plate, it is important to only flame the loop before collecting a bacterial
sample, not for every subsequent streak
True / False? - CORRECT ANSWERS False
What is a bacterial colony? - CORRECT ANSWERS a collection of bacterial cells that arose from
a single cell
, Why is the use of oil immersion important when the 100x objective lens is in place? - CORRECT
ANSWERS it allows more light rays to enter directly into the objective lens to produce a clearer
image
Which part of the microscope magnifies the specimen at various magnifications? - CORRECT
ANSWERS objective lens
The ability to distinguish between two distinct points, which determines clarity of an image, is known
as... - CORRECT ANSWERS resolving power
What property of the microscopes used in lab allows us to maintain coarse focus at 400x total
magnification after having obtained focus a the lowest power objective? - CORRECT
ANSWERS parfocal
When looking through the ocular lens, you will see... - CORRECT ANSWERS the field of views
Which of the following is/are important when preparing a bacterial smear?
(a) thin smears
(b) complete air-drying prior to heat fixation
(c) cleaning slides
(d) all of the above - CORRECT ANSWERS all of the above
Mesophiles have an optimum growth temperature at around...
(a) 25 C
(b) 37 C
(c) 60 C
(d) 100 C - CORRECT ANSWERS (b) 37 C
Bacterial that are incubated below their optimum growth temperature would... - CORRECT
ANSWERS exhibit decreased growth rates
CORRECT Answers
Aseptic technique includes...
(a) sterilization of transfer tools before and after use
(b) disinfection of bench tops
(c) washing hands
(d) all of the above - CORRECT ANSWERS (d) all of the above
Placing the cap of a broth cultures up on the bench top is acceptable
True/False? - CORRECT ANSWERS False
Why is it important to allow the inoculating loop to cool prior to obtaining the inoculum? - CORRECT
ANSWERS to avoid frying the cell
A pure culture can be prepared from a mixed broth or a mixed agar slant culture directly
True/False - CORRECT ANSWERS False
Which of the following is a benefit of using the streak plate method for obtaining isolated colonies
compared to the pour plate method?
(a) the streak plate method allows you to observe colonies on the surface and deep in the agar
(b) the pour plate method does not require serial dilutions
(c) the streak plate method requires serial dilutions
(d) the streak plate method does not require serial dilutions - CORRECT ANSWERS (d) the
streak plate method does not require serial dilutions
When preparing a streak plate, it is important to only flame the loop before collecting a bacterial
sample, not for every subsequent streak
True / False? - CORRECT ANSWERS False
What is a bacterial colony? - CORRECT ANSWERS a collection of bacterial cells that arose from
a single cell
, Why is the use of oil immersion important when the 100x objective lens is in place? - CORRECT
ANSWERS it allows more light rays to enter directly into the objective lens to produce a clearer
image
Which part of the microscope magnifies the specimen at various magnifications? - CORRECT
ANSWERS objective lens
The ability to distinguish between two distinct points, which determines clarity of an image, is known
as... - CORRECT ANSWERS resolving power
What property of the microscopes used in lab allows us to maintain coarse focus at 400x total
magnification after having obtained focus a the lowest power objective? - CORRECT
ANSWERS parfocal
When looking through the ocular lens, you will see... - CORRECT ANSWERS the field of views
Which of the following is/are important when preparing a bacterial smear?
(a) thin smears
(b) complete air-drying prior to heat fixation
(c) cleaning slides
(d) all of the above - CORRECT ANSWERS all of the above
Mesophiles have an optimum growth temperature at around...
(a) 25 C
(b) 37 C
(c) 60 C
(d) 100 C - CORRECT ANSWERS (b) 37 C
Bacterial that are incubated below their optimum growth temperature would... - CORRECT
ANSWERS exhibit decreased growth rates
