SCYM (ASCP) FLOW CYTOMETRY
QUIZLET 2WITH CORRECT ANSWERS
GRADED A+ FOR 2026 ASSURED
EXCEL!!
FLOW CYTOMETRY MASTER REVISION GUIDE
Comprehensive Exam Questions & Correct Answers for Medical Laboratory Science,
Hematology, Immunology & Clinical Diagnostics
1. Hydrodynamic Focusing
CORRECT ANSWER: Most modern flow cytometers tightly position the sample for optical
analysis via hydrodynamic focusing. A carrier fluid called the sheath fluid positions cells into a
single file for optical interrogation.
2. Hydrodynamic Focusing and Sheath Fluids
CORRECT ANSWER: The central sample stream is focused and surrounded by the slower sheath
fluid. The flow cell shape is crucial and is traditionally nozzle-shaped. The sheath fluid pressure
remains constant while the sample fluid pressure is adjusted.
3. Sample Pressure and Sheath Pressure
CORRECT ANSWER: The difference between sample pressure and sheath pressure is called the
differential pressure. It controls the width of the core stream and the number of cells passing
through the laser intercept.
4. Differential Pressure-Based Flow Cytometers
CORRECT ANSWER: These systems dominate the market and contain two pressure regulators:
1. Constant pressure regulator controlling fluid speed.
2. Adjustable regulator controlled by the operator.
5. Generation of Differential Pressure (Syringe Pump, Pressure-Based)
, CORRECT ANSWER: Low differential pressure allows cells to pass one at a time through the
interrogation point. Pressure can be generated using pump and regulator systems, including
peristaltic and syringe pumps.
6. Characterization of Syringe-Pump-Driven Induced Pressure
CORRECT ANSWER: Syringe-pump-driven systems may experience pressure fluctuations due to
mechanical oscillations generated by the stepper motor, resulting in flow-rate and pressure
variations.
7. Optical Filters
CORRECT ANSWER: Optical filters are specially coated glass components that allow selected
wavelengths to pass while blocking others. Types include:
1. Bandpass filters
2. Longpass filters
3. Shortpass filters
8. Band Pass Optical Filter
CORRECT ANSWER: Allows light within a specific wavelength range to pass and reflects
wavelengths above and below that range. Example: A 550/40 nm filter passes wavelengths from
530–570 nm.
9. Longpass Filter
CORRECT ANSWER: Transmits wavelengths above 650 nm.
10. Shortpass Filter
CORRECT ANSWER: Transmits wavelengths below 488 nm.
11. Dichroic Mirrors
CORRECT ANSWER: Dichroic mirrors separate light by transmitting specific wavelengths while
reflecting others. Example:
• 500LP transmits light above 500 nm.
• 525SP transmits light below 525 nm.
12. Neutral Density Filter
CORRECT ANSWER: Reduces the intensity of all wavelengths equally without altering color.
QUIZLET 2WITH CORRECT ANSWERS
GRADED A+ FOR 2026 ASSURED
EXCEL!!
FLOW CYTOMETRY MASTER REVISION GUIDE
Comprehensive Exam Questions & Correct Answers for Medical Laboratory Science,
Hematology, Immunology & Clinical Diagnostics
1. Hydrodynamic Focusing
CORRECT ANSWER: Most modern flow cytometers tightly position the sample for optical
analysis via hydrodynamic focusing. A carrier fluid called the sheath fluid positions cells into a
single file for optical interrogation.
2. Hydrodynamic Focusing and Sheath Fluids
CORRECT ANSWER: The central sample stream is focused and surrounded by the slower sheath
fluid. The flow cell shape is crucial and is traditionally nozzle-shaped. The sheath fluid pressure
remains constant while the sample fluid pressure is adjusted.
3. Sample Pressure and Sheath Pressure
CORRECT ANSWER: The difference between sample pressure and sheath pressure is called the
differential pressure. It controls the width of the core stream and the number of cells passing
through the laser intercept.
4. Differential Pressure-Based Flow Cytometers
CORRECT ANSWER: These systems dominate the market and contain two pressure regulators:
1. Constant pressure regulator controlling fluid speed.
2. Adjustable regulator controlled by the operator.
5. Generation of Differential Pressure (Syringe Pump, Pressure-Based)
, CORRECT ANSWER: Low differential pressure allows cells to pass one at a time through the
interrogation point. Pressure can be generated using pump and regulator systems, including
peristaltic and syringe pumps.
6. Characterization of Syringe-Pump-Driven Induced Pressure
CORRECT ANSWER: Syringe-pump-driven systems may experience pressure fluctuations due to
mechanical oscillations generated by the stepper motor, resulting in flow-rate and pressure
variations.
7. Optical Filters
CORRECT ANSWER: Optical filters are specially coated glass components that allow selected
wavelengths to pass while blocking others. Types include:
1. Bandpass filters
2. Longpass filters
3. Shortpass filters
8. Band Pass Optical Filter
CORRECT ANSWER: Allows light within a specific wavelength range to pass and reflects
wavelengths above and below that range. Example: A 550/40 nm filter passes wavelengths from
530–570 nm.
9. Longpass Filter
CORRECT ANSWER: Transmits wavelengths above 650 nm.
10. Shortpass Filter
CORRECT ANSWER: Transmits wavelengths below 488 nm.
11. Dichroic Mirrors
CORRECT ANSWER: Dichroic mirrors separate light by transmitting specific wavelengths while
reflecting others. Example:
• 500LP transmits light above 500 nm.
• 525SP transmits light below 525 nm.
12. Neutral Density Filter
CORRECT ANSWER: Reduces the intensity of all wavelengths equally without altering color.
