ASCP SCYM study guide with questions and correct answers graded a+ for 2026 assured excel!!

ASCP SCYM study guide with
questions and correct answers graded
a+ for 2026 assured excel!!

FLOW CYTOMETRY MASTER REVISION GUIDE – PART 2

Clinical Applications, Immunophenotyping, Quality Control & Laboratory Management



SAMPLE PROCESSING

1. Sample Processing

CORRECT ANSWER:

• Optimized antibody concentration

• Defined range of cell numbers

• Defined sample handling procedures

• Controlled time and temperature

• Defined acceptable delay before acquisition



IMPORTANT CD MARKERS

Marker CORRECT ANSWER

CD2 T Cells, NK Cells

CD3 T Cells

CD4 T Helper Cells, Monocytes (dim)

CD5 T Cells, Some B Cells

CD8 Cytotoxic T Cells, Macrophages

, Marker CORRECT ANSWER

CD10 Immature T Cells, B Cells, PMNs

CD13 Monocytes, PMNs

CD14 Monocytes, PMNs (dim)

CD16 NK Cells, PMNs, Monocytes

CD19 B Cells

CD20 B Cells

CD25 Activated T Cells

CD33 Monocytes

CD34 Stem/Progenitor Cells

CD45 Leukocytes

CD56 NK Cells, Monocytes (dim)

Anti-Kappa B Cells

Anti-Lambda B Cells

TdT Immature Lymphocytes



MONOCLONAL ANTIBODY STAINING

2. Direct Staining

CORRECT ANSWER:

• Rapid single-step staining

• Multiple antibodies from the same host can be used

3. Indirect Staining

CORRECT ANSWER:

• Secondary antibody amplifies signal