BIOD 171 LABORATORY EXAMS
2026 2027 VERIFIED ANSWERS
ACTUAL UPDATED PRACTICE
QUESTIONS HIGH YIELD
STUDY GUIDE EXAM PREP
MATERIAL LATEST VERSION
GRADED A+ SUCCESS REVIEW
PACK COMPLETE EDITION
FINAL RELEASE LAB
RESOURCE
LAB VIII
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
,
,The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step
D (above)?
All of the wells would have a dark color. The intensity of the readout is dependent upon the
amount of the labeled secondary antibody present in the well. Since the unbound secondary
antibody was not washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step post-transfer?
Blocking prevents non-specific binding of the antibody—you only want the antibody to bind
to its specific target.
3. When developing a western blot what two factors influence the intensity of the band?
Both the amount of protein loaded as well as the duration (time) of development is directly
proportional to band intensity.
,
, ,
BIOD 171: LABORATORY EXAMS
QUESTIONS AND ANSWERS CERTIFIED
100% 2026 UPDATED VERSION
4. Identify (specific name) given to the region of the western blot indicated by the arrow.
The arrow is pointing to the dye front.
,
, ,
BIOD 171: LABORATORY EXAMS
QUESTIONS AND ANSWERS CERTIFIED
100% 2026 UPDATED VERSION
1. What is the common name for agglutination?
Clumping
,
2026 2027 VERIFIED ANSWERS
ACTUAL UPDATED PRACTICE
QUESTIONS HIGH YIELD
STUDY GUIDE EXAM PREP
MATERIAL LATEST VERSION
GRADED A+ SUCCESS REVIEW
PACK COMPLETE EDITION
FINAL RELEASE LAB
RESOURCE
LAB VIII
1. The word ‘antigen’ is actually a combination for what two words?
Antibody-generating
2. For the ELISA assay depicted below, what do the arrows indicate?
,
,The arrows indicate the wash steps.
3. What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step
D (above)?
All of the wells would have a dark color. The intensity of the readout is dependent upon the
amount of the labeled secondary antibody present in the well. Since the unbound secondary
antibody was not washed away, all wells would react equally.
1. In a Western blot are separated based on its size.
proteins
2. When setting up a western blot, what is the purpose of the blocking step post-transfer?
Blocking prevents non-specific binding of the antibody—you only want the antibody to bind
to its specific target.
3. When developing a western blot what two factors influence the intensity of the band?
Both the amount of protein loaded as well as the duration (time) of development is directly
proportional to band intensity.
,
, ,
BIOD 171: LABORATORY EXAMS
QUESTIONS AND ANSWERS CERTIFIED
100% 2026 UPDATED VERSION
4. Identify (specific name) given to the region of the western blot indicated by the arrow.
The arrow is pointing to the dye front.
,
, ,
BIOD 171: LABORATORY EXAMS
QUESTIONS AND ANSWERS CERTIFIED
100% 2026 UPDATED VERSION
1. What is the common name for agglutination?
Clumping
,
