A LEVEL BIOLOGY B: Actual
June 2025 PAST PAPER 3:
General and Practical Principles
in Biology. All Assessment
Questions & Mark Scheme
[Edexcel 9BI0/03]
,A LEVEL BIOLOGY B: Actual June 2025 PAST PAPER 3: General and Practical Principles in
Biology. All Assessment Questions & Mark Scheme [Edexcel 9BI0/03]
Exam Resource Summary
The June 2025 Edexcel A Level Biology B 9BIO/03 – Paper 3: General and Practical Principles in
Biology – Merged Question Paper & Mark Scheme document combines the official examination paper
with its full mark scheme. This paper evaluates students’ understanding of experimental
techniques, practical skills, and general biological principles, including data analysis, experimental
design, and the application of theory to real-world biological contexts. The merged format presents
each question alongside its marking criteria, providing clear guidance on examiner expectations,
mark allocation, and question structure. This resource enables targeted revision, development of
analytical and evaluative skills, and practice in interpreting and applying biological data, making it
essential for preparation for the June 2025 Edexcel A Level Biology B Paper 3 examination.
Turn over
, Answer ALL questions.
Write your answers in the spaces provided.
AREA
D O NOT WRITE IN THIS
1 Saccharomyces cerevisiae (yeast) is a single-celled organism widely used in brewing
and baking.
The diagram shows a drawing of a yeast cell, as seen using an electron microscope.
A cytoplasm containing
cell surface glycogen granules
membrane
nucleus containing 16
linear chromosomes
cell wall made of
chitin and mannose
AREA
D O NOT WRITE IN THIS
vacuole
mitochondrion
B
(a) The actual size of the cell measured from point A to point B is 6 µm.
Calculate the magnification of this yeast cell.
Give your answer to the nearest whole number.
(2)
AREA
D O NOT WRITE IN THIS
Answer ..............................................................
2
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, (b) Draw this yeast cell as it would be seen using a light microscope at
high magnification.
DO NOT WRITE IN THIS AREA
Labels are not required.
(2)
DO NOT WRITE IN THIS AREA
(c) The information in the diagram can be used to classify yeast.
(i) State why yeast is classified as eukaryotic not prokaryotic.
(1)
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . . . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
DO NOT WRITE IN THIS AREA
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
(ii) State why yeast is not classified as a plant.
(1)
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . . . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
(Total for Question 1 = 6 marks)
3
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June 2025 PAST PAPER 3:
General and Practical Principles
in Biology. All Assessment
Questions & Mark Scheme
[Edexcel 9BI0/03]
,A LEVEL BIOLOGY B: Actual June 2025 PAST PAPER 3: General and Practical Principles in
Biology. All Assessment Questions & Mark Scheme [Edexcel 9BI0/03]
Exam Resource Summary
The June 2025 Edexcel A Level Biology B 9BIO/03 – Paper 3: General and Practical Principles in
Biology – Merged Question Paper & Mark Scheme document combines the official examination paper
with its full mark scheme. This paper evaluates students’ understanding of experimental
techniques, practical skills, and general biological principles, including data analysis, experimental
design, and the application of theory to real-world biological contexts. The merged format presents
each question alongside its marking criteria, providing clear guidance on examiner expectations,
mark allocation, and question structure. This resource enables targeted revision, development of
analytical and evaluative skills, and practice in interpreting and applying biological data, making it
essential for preparation for the June 2025 Edexcel A Level Biology B Paper 3 examination.
Turn over
, Answer ALL questions.
Write your answers in the spaces provided.
AREA
D O NOT WRITE IN THIS
1 Saccharomyces cerevisiae (yeast) is a single-celled organism widely used in brewing
and baking.
The diagram shows a drawing of a yeast cell, as seen using an electron microscope.
A cytoplasm containing
cell surface glycogen granules
membrane
nucleus containing 16
linear chromosomes
cell wall made of
chitin and mannose
AREA
D O NOT WRITE IN THIS
vacuole
mitochondrion
B
(a) The actual size of the cell measured from point A to point B is 6 µm.
Calculate the magnification of this yeast cell.
Give your answer to the nearest whole number.
(2)
AREA
D O NOT WRITE IN THIS
Answer ..............................................................
2
■■■■
, (b) Draw this yeast cell as it would be seen using a light microscope at
high magnification.
DO NOT WRITE IN THIS AREA
Labels are not required.
(2)
DO NOT WRITE IN THIS AREA
(c) The information in the diagram can be used to classify yeast.
(i) State why yeast is classified as eukaryotic not prokaryotic.
(1)
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . . . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
DO NOT WRITE IN THIS AREA
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
(ii) State why yeast is not classified as a plant.
(1)
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . . . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. .. . . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . .. . . ......................
(Total for Question 1 = 6 marks)
3
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