DNA typing of urine
Give this one a try later!
•There are few cells available in urine for DNA analysis •Liquid urine samples
have a better rate of success for DNA discovery, but rate is still very low when
compared to other methods for DNA analysis. Urine stains, which have low
cell concentrations, are even lower. •Bacterial content of urine may inhibit
successful DNA Analysis
Acid phosphates
Give this one a try later!
, •Seminal Acid phosphatase •( SAP) is an enzyme that can catalyze the
hydrolysis of certain organic phosphate
Identifying semen
No variation of SAP between males with normal sperm counts and infertile
male
Limitations of amylose
Give this one a try later!
•Using amylase as indicator for saliva is limited by presence of amylase in:
Feces via pancreas Perspiration Vaginal secretions Semen Combinations of
these fluids
•Phadebas™ test is another test for amylase but this test may not detect low
levels of amylase
Other body fluids
Give this one a try later!
Urine detection •relies on two organic compounds - urea (present in high
levels) and creatinine (present at 1/10 level of urea)
•Testing for urine relies on use of urease, an enzyme which breaks down and
releases ammonia and carbon dioxid
Vaginal secretions
Give this one a try later!
Give this one a try later!
•There are few cells available in urine for DNA analysis •Liquid urine samples
have a better rate of success for DNA discovery, but rate is still very low when
compared to other methods for DNA analysis. Urine stains, which have low
cell concentrations, are even lower. •Bacterial content of urine may inhibit
successful DNA Analysis
Acid phosphates
Give this one a try later!
, •Seminal Acid phosphatase •( SAP) is an enzyme that can catalyze the
hydrolysis of certain organic phosphate
Identifying semen
No variation of SAP between males with normal sperm counts and infertile
male
Limitations of amylose
Give this one a try later!
•Using amylase as indicator for saliva is limited by presence of amylase in:
Feces via pancreas Perspiration Vaginal secretions Semen Combinations of
these fluids
•Phadebas™ test is another test for amylase but this test may not detect low
levels of amylase
Other body fluids
Give this one a try later!
Urine detection •relies on two organic compounds - urea (present in high
levels) and creatinine (present at 1/10 level of urea)
•Testing for urine relies on use of urease, an enzyme which breaks down and
releases ammonia and carbon dioxid
Vaginal secretions
Give this one a try later!
