Howdoes Chromatography work
Components dissolve in mobile phase I movethrough thestationary piased
How
fastcompoundsgetcarried up theplate orthrough columndepends on
1 Howsolublethecompound is in thesolvent optionbehome
Ifield to
2 Howmuchthecompound sticks tothestationaryphase
3 Theattraction betweenthemolecules being separated l the Mesffharythese
mobile stationaryphase depends on nature intermolecular
forces in each
of
Mobile Phase
THIS graphy
Stationary Phase
Paper Solid paper liquid solvent
Thin layer
Chromatography Solid Silica Liquid solvent
Gas Liquid
chromatography liquid sushi gas
ineliggy
Paper Chromatography TLC
Methodfor Paper 1 Thin layer Chromatography
Drawbaseline using a pencilclosetobottom
SpotsampleontoTLC plate
of TLCplate
usingcapillarytube
PlaceTLC plate in beakerwith Shallow amount
newtopofTLCplate
of solvent I allowsolventtorun upuntil
Marksolventfront I allow to dry
Use locatingagent like ninhydrin or UVlight to identifyspots
Work out Rg
Extra
if asked how do you tellthe rxn is complete
If spotofsample is no longerpresent then rxn is complete
to compare purity samesteps butinstead
use a polarsolvent
ofspoteinstead ofRg
Compare no
, used I not a pen
Whyis aispencil
insoluble in water solventso won't
pencil affect results
using a capillary tube
are spotsapplied
Why
allowssmall ofsample to bedeposited
amounts
prevents overloading
accurate I clear results
Whyseveralspotsofthemixture are applied in thesameplace
to allow a concentated Sample
Whythesolute must beallowed toevaporate before applying subsequent spots
to concentrate thesample l preventinterference withthe mobile phase solvent
Whythesolventlevel mustbe belowthe pencil line
So sampledoesn't dissolveintotheexcesssolvent
Why the chromatography shouldalways becarried outin a closed container
to prevent thesolventfrom evaporating usually volitile liquids
Whythepositionofthesolventfront mustbemarked assoonas the chromatogram is
removedfromthetank
as thesolvent will evaporate l thelinewillfadeover time
Whymust you wear gloves when handlingchromatography paper ofaminoacids
proteins aminoacids protease on theskin will affect the sample
Components dissolve in mobile phase I movethrough thestationary piased
How
fastcompoundsgetcarried up theplate orthrough columndepends on
1 Howsolublethecompound is in thesolvent optionbehome
Ifield to
2 Howmuchthecompound sticks tothestationaryphase
3 Theattraction betweenthemolecules being separated l the Mesffharythese
mobile stationaryphase depends on nature intermolecular
forces in each
of
Mobile Phase
THIS graphy
Stationary Phase
Paper Solid paper liquid solvent
Thin layer
Chromatography Solid Silica Liquid solvent
Gas Liquid
chromatography liquid sushi gas
ineliggy
Paper Chromatography TLC
Methodfor Paper 1 Thin layer Chromatography
Drawbaseline using a pencilclosetobottom
SpotsampleontoTLC plate
of TLCplate
usingcapillarytube
PlaceTLC plate in beakerwith Shallow amount
newtopofTLCplate
of solvent I allowsolventtorun upuntil
Marksolventfront I allow to dry
Use locatingagent like ninhydrin or UVlight to identifyspots
Work out Rg
Extra
if asked how do you tellthe rxn is complete
If spotofsample is no longerpresent then rxn is complete
to compare purity samesteps butinstead
use a polarsolvent
ofspoteinstead ofRg
Compare no
, used I not a pen
Whyis aispencil
insoluble in water solventso won't
pencil affect results
using a capillary tube
are spotsapplied
Why
allowssmall ofsample to bedeposited
amounts
prevents overloading
accurate I clear results
Whyseveralspotsofthemixture are applied in thesameplace
to allow a concentated Sample
Whythesolute must beallowed toevaporate before applying subsequent spots
to concentrate thesample l preventinterference withthe mobile phase solvent
Whythesolventlevel mustbe belowthe pencil line
So sampledoesn't dissolveintotheexcesssolvent
Why the chromatography shouldalways becarried outin a closed container
to prevent thesolventfrom evaporating usually volitile liquids
Whythepositionofthesolventfront mustbemarked assoonas the chromatogram is
removedfromthetank
as thesolvent will evaporate l thelinewillfadeover time
Whymust you wear gloves when handlingchromatography paper ofaminoacids
proteins aminoacids protease on theskin will affect the sample
