BIOCHEMISTRY ACS EXAM SCRIPT 2026 QUESTIONS WITH DETAILED ANSWERS GRADED A+

BIOCHEMISTRY ACS EXAM SCRIPT 2026
QUESTIONS WITH DETAILED ANSWERS
GRADED A+



◉what step produces 2x NADH in glycolysis?.ANSWER:-G3P -> 1,3
BPG


◉What enzyme/pathway does ATP inhibit?.ANSWER:-A high level
of ATP inhibits the enzyme PFK-1 by decreasing its affinity for
fructose 6-phosphate. AMP diminishes and citrate enhances the
inhibitory effect of ATP.


◉The NADPH produced by HMP is used for.ANSWER:-reductive
biosynthesis (anabolic)


◉how do competitive inhibitors affect Km and Vmax?.ANSWER:-
increases Km, doesn't change Vmax


◉how do noncompetitive inhibitors affect Km and Vmax?.ANSWER:-
Km doesn't change, Vmax lowerse


◉SDS-PAGE.ANSWER:-Type of chromatography used to separate
proteins based on mass. SDS binds to the proteins, giving them a

, negative charge cancelling the effect of charges from the individual
amino acids. Lighter proteins travel faster than heavier ones.


◉PCR.ANSWER:-polymerase chain reaction- DNA is copied multiple
times to produce many copies of the original molecules helpful
when there's only a small DNA sample


◉Arginine (pka).ANSWER:-Positively charged R groups (pKa=12.5)


◉Aspartic Acid (pka).ANSWER:-Negatively charged R groups
(pKa=3.9)


◉Cysteine (pka).ANSWER:-Polar, uncharged R groups (pKa=8.3)


◉Glutamic Acid (pka).ANSWER:-Negatively charged R groups
(pKa=4.3)


◉Histidine (pka).ANSWER:-Positively charged R groups (pKa=6.0)


◉Lysine (pka).ANSWER:-Positively charged R groups (pKa=10.5)


◉Tyrosine (pka).ANSWER:-Positively charged R groups (pKa=10.1)