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MIP 300 Exam 1 TEST FINAL EXAM AND PRACTICE EXAM
2026/2027 BANK 2 VERSIONS QUESTIONS WITH DETAILED
VERIFIED ANSWERS / EXAM QUESTIONS WILL COME
FROM HERE (100% CORRECT ANSWERS/ A+ GRADED
Microbe - ANSWERS--Acellular or cellular objects that cannot typically
be visualized with the naked eye
Virus - ANSWERS--Acellular microbe made of proteins and DNA or RNA,
uses certain types of host cells to reproduce and develop infectious
characteristics
Prion - ANSWERS--Acellular microbe made of a misfolded protein, can
become infectious depending on the misfolding causing other proteins
to misfold and become infectious prions as well
Bacteria - ANSWERS--Unicellular microbes considered prokaryotic, have
no nucleus or membrane bound organelles ex) e. coli
Archaea - ANSWERS--Unicellular microbes considered prokaryotic but
differentiated at a later point from eukaryotic cell development from
eubacteria, have no nucleus or membrane bound organelles ex)
thermus aquaticus
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Algae - ANSWERS--Unicellular colonial microbes considered eukaryotic
protists, have nucleus and membrane bound organelles ex) Protafeca
Protozoa - ANSWERS--Unicellular microbes considered transitional
eukaryotic protists, have varying development of membrane bound
organelles ex) giardia
Yeast - ANSWERS--Unicellular microbes considered eukaryotic fungi,
have nucleus and membrane bound organelles
Molds - ANSWERS--Uni/multicellular microbes considered eukaryotic
fungi, have nucleus and membrane bound organelles
5 Kingdom system (outdated) - ANSWERS--1) Monera - bacteria,
archaea
2) Protista - Protozoa, algae
3) Fungi - Yeast, mold, mushroom
4) Plantae - Trees and shit
5) Animalia - Dogs and shit
3 Domains system (more accurate) - ANSWERS--1) Bacteria
2) Archaea
3) Eukarya
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Naming/classifying bacteria, fungi, and protozoa - ANSWERS--Genus,
species, strain ex) Escheria coli 15H7 (underlined)
Magnification= - ANSWERS--Objective x Eyepiece
Resolving power - ANSWERS--Ability of lens to distinguish between
objects close together
Light microscopy - ANSWERS--Resolution power of .2 um, can see
eukaryotes and prokaryotes but not viruses
4 types of light microscopy - ANSWERS--1) Bright field - Must heat fix
(kill) and stain first, background light, bacteria appear as color of stain
2) Dark field - Light refracted by organism, background dark, organism
bright
3) Phase contrast - converts slight differences in refractive indices to be
easily detected variables
4) Fluorescence - Uses UV light to excite fluorescent molecules such as
GFP or fluorochime labelled antibodies
Electron microscopy - ANSWERS--Resolution 1000x better than light
microscope - .0005 um resolution
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Basic dyes - ANSWERS--Bind to negatively charged structures
Acidic dyes - ANSWERS--Bind to positively charged structures
Differential stains - ANSWERS--Divide microbes into caegories
depending on structural characteristics
Gram stains - ANSWERS--Separate bacteria based on type of cell wall (+
or - charge)
Gram positive stain - ANSWERS--Indicates a thick cell wall
Gram negative stain - ANSWERS--Indicates a thin cell wall
Acid fast stain - ANSWERS--Type of differential stain that identifies
bacteria neither pos. or neg. that have thick waxy mycolic acid layer
around them, have to use heat to get stain to stick
Steps in an acid fast stain - ANSWERS--1) Addition of carbol fushia
2) Heating of slide
3) Acid alcohol wash
4) Counterstain
MIP 300 Exam 1 TEST FINAL EXAM AND PRACTICE EXAM
2026/2027 BANK 2 VERSIONS QUESTIONS WITH DETAILED
VERIFIED ANSWERS / EXAM QUESTIONS WILL COME
FROM HERE (100% CORRECT ANSWERS/ A+ GRADED
Microbe - ANSWERS--Acellular or cellular objects that cannot typically
be visualized with the naked eye
Virus - ANSWERS--Acellular microbe made of proteins and DNA or RNA,
uses certain types of host cells to reproduce and develop infectious
characteristics
Prion - ANSWERS--Acellular microbe made of a misfolded protein, can
become infectious depending on the misfolding causing other proteins
to misfold and become infectious prions as well
Bacteria - ANSWERS--Unicellular microbes considered prokaryotic, have
no nucleus or membrane bound organelles ex) e. coli
Archaea - ANSWERS--Unicellular microbes considered prokaryotic but
differentiated at a later point from eukaryotic cell development from
eubacteria, have no nucleus or membrane bound organelles ex)
thermus aquaticus
,2|Page
Algae - ANSWERS--Unicellular colonial microbes considered eukaryotic
protists, have nucleus and membrane bound organelles ex) Protafeca
Protozoa - ANSWERS--Unicellular microbes considered transitional
eukaryotic protists, have varying development of membrane bound
organelles ex) giardia
Yeast - ANSWERS--Unicellular microbes considered eukaryotic fungi,
have nucleus and membrane bound organelles
Molds - ANSWERS--Uni/multicellular microbes considered eukaryotic
fungi, have nucleus and membrane bound organelles
5 Kingdom system (outdated) - ANSWERS--1) Monera - bacteria,
archaea
2) Protista - Protozoa, algae
3) Fungi - Yeast, mold, mushroom
4) Plantae - Trees and shit
5) Animalia - Dogs and shit
3 Domains system (more accurate) - ANSWERS--1) Bacteria
2) Archaea
3) Eukarya
,3|Page
Naming/classifying bacteria, fungi, and protozoa - ANSWERS--Genus,
species, strain ex) Escheria coli 15H7 (underlined)
Magnification= - ANSWERS--Objective x Eyepiece
Resolving power - ANSWERS--Ability of lens to distinguish between
objects close together
Light microscopy - ANSWERS--Resolution power of .2 um, can see
eukaryotes and prokaryotes but not viruses
4 types of light microscopy - ANSWERS--1) Bright field - Must heat fix
(kill) and stain first, background light, bacteria appear as color of stain
2) Dark field - Light refracted by organism, background dark, organism
bright
3) Phase contrast - converts slight differences in refractive indices to be
easily detected variables
4) Fluorescence - Uses UV light to excite fluorescent molecules such as
GFP or fluorochime labelled antibodies
Electron microscopy - ANSWERS--Resolution 1000x better than light
microscope - .0005 um resolution
, 4|Page
Basic dyes - ANSWERS--Bind to negatively charged structures
Acidic dyes - ANSWERS--Bind to positively charged structures
Differential stains - ANSWERS--Divide microbes into caegories
depending on structural characteristics
Gram stains - ANSWERS--Separate bacteria based on type of cell wall (+
or - charge)
Gram positive stain - ANSWERS--Indicates a thick cell wall
Gram negative stain - ANSWERS--Indicates a thin cell wall
Acid fast stain - ANSWERS--Type of differential stain that identifies
bacteria neither pos. or neg. that have thick waxy mycolic acid layer
around them, have to use heat to get stain to stick
Steps in an acid fast stain - ANSWERS--1) Addition of carbol fushia
2) Heating of slide
3) Acid alcohol wash
4) Counterstain
