Ch 2 - Cowan, Microbiology Fundamentals: A Clinical Approach, 3e
Study online at https://quizlet.com/_6x6vxb
1. The 5 I's: 1. Inoculation
2. Incubation
3. Isolation
4. Inspection
5. Identification
2. Inoculation: sample is placed into a container of growth medium via inoculation. medium can be solid or liquid
or a live animal such as chicken embryo
3. Incubation: an incubator creates the proper growth conditions with respect to temperature and gas require-
ments
4. Isolation: Once the cultures have grown, they may need to be re-inoculated (and incubated) in such a way that
separate species are obtained
5. Inspection: the colonies on agar or the broth cultures are observed macroscopically and microscopically,
possibly with the aid of staining
6. Identification: the identity of the isolated microbe is determined, usually to the species level. Inspection may
be enough to identify some microbes, but additional techniques include biochemical tests, immunologic tests, and
genetic analysis.
7. culture: the visible accumulation of microorganisms in or on a nutrient medium.
the propagation of microorganisms with various media
8. medium: plural - media. nutrient used to grow organisms outside their natural habitats
9. most common manifestation of growth on solid media: colonies
10. Media: 3 properties: physical state, chemical composition, functional type (purpose)
11. Physical state of media: liquid, semisolid, solid (that can be converted to liquid), solid (that cannot be
liquefied
12. Chemical composition of media: synthetic (chemically defined) and non synthetic (complex)
13. Functional type of media: general purpose, enriched, selective, differential, anaerobic growth, speci-
men transport, assay, enumeration
14. Defined (synthetic) media: media whose chemical compositions are precisely chemically defined
15. Complex media: extracts and digests of yeasts, meat, or plants
16. General-purpose media: to grow as broad a spectrum of microbes as possible
Examples: nutrient agar and broth, brain-heart infusion, trypticase soy agar (TSA)
17. Enriched medium: Contains complex organic substances such as blood, serum, hemoglobin, or special
growth factors (specific vitamins, amino acids), that certain species must have in order to grow.
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Study online at https://quizlet.com/_6x6vxb
1. The 5 I's: 1. Inoculation
2. Incubation
3. Isolation
4. Inspection
5. Identification
2. Inoculation: sample is placed into a container of growth medium via inoculation. medium can be solid or liquid
or a live animal such as chicken embryo
3. Incubation: an incubator creates the proper growth conditions with respect to temperature and gas require-
ments
4. Isolation: Once the cultures have grown, they may need to be re-inoculated (and incubated) in such a way that
separate species are obtained
5. Inspection: the colonies on agar or the broth cultures are observed macroscopically and microscopically,
possibly with the aid of staining
6. Identification: the identity of the isolated microbe is determined, usually to the species level. Inspection may
be enough to identify some microbes, but additional techniques include biochemical tests, immunologic tests, and
genetic analysis.
7. culture: the visible accumulation of microorganisms in or on a nutrient medium.
the propagation of microorganisms with various media
8. medium: plural - media. nutrient used to grow organisms outside their natural habitats
9. most common manifestation of growth on solid media: colonies
10. Media: 3 properties: physical state, chemical composition, functional type (purpose)
11. Physical state of media: liquid, semisolid, solid (that can be converted to liquid), solid (that cannot be
liquefied
12. Chemical composition of media: synthetic (chemically defined) and non synthetic (complex)
13. Functional type of media: general purpose, enriched, selective, differential, anaerobic growth, speci-
men transport, assay, enumeration
14. Defined (synthetic) media: media whose chemical compositions are precisely chemically defined
15. Complex media: extracts and digests of yeasts, meat, or plants
16. General-purpose media: to grow as broad a spectrum of microbes as possible
Examples: nutrient agar and broth, brain-heart infusion, trypticase soy agar (TSA)
17. Enriched medium: Contains complex organic substances such as blood, serum, hemoglobin, or special
growth factors (specific vitamins, amino acids), that certain species must have in order to grow.
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