TEST BANK Microbiology with Diseases by Body System By
Robert Bauman 5th Edition All Chapters Included With Complete
Solutions 2026/2027
Chapter : Recombinant DNA Technology
8.1 Multiple Choice Questions
1) The use of microorganisms to produce useful products is known as
A) biotechnology.
B) gene modification.
C) genomics.
D) recombinant DNA technology.
E) cloning.
Answer: A
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.1
2) In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make
for the first time in an industrial setting.
A) soy sauce
B) acetone
C) wine
D) cheese
E) alcohol
Answer: B
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.2
3) Which of the following items is NOT a part of the name of a restriction enzyme?
A) the Gram reaction of the source bacterium
B) the specific epithet of the source bacterium
C) Roman numerals to indicate its order of discovery
D) the strain of the source bacterium
E) the genus of the source bacterium
Answer: A
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.8
,4) The natural role of restriction enzymes in bacteria is to
A) make conjugation more efficient.
B) allow transposons to move to another place in the chromosome.
C) protect the cell from invading phages.
D) allow cells to accept foreign DNA.
E) provide the cell with new phenotypes, such as antibiotic resistance.
Answer: C
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9
5) Which of the following is a product of recombinant DNA technology?
A) rice that produces vitamin C
B) bacteria that produce insulin
C) yeast that produces alcohol
D) bacteria that produce acetone
E) microbes that digest cellulose
Answer: B
Bloom's Taxonomy: Application
Section: Applications of Recombinant DNA Technology
Learning Outcome: 8.20
6) Which of the following restriction enzyme sites would produce blunt-ended fragments? (The
arrow represents the cutting site of the enzyme.)
A) C↓CGG
B) G↓GATCC
C) G↓AATTC
D) CCC↓GGG
E) A↓AGCTT
Answer: D
Bloom's Taxonomy: Application
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9
7) The enzyme reverse transcriptase
A) synthesizes DNA to repair gaps in a DNA strand.
B) synthesizes DNA from an RNA template.
C) synthesizes RNA from an RNA template.
D) synthesizes RNA from a DNA template.
E) can synthesize DNA or RNA from an RNA template.
Answer: B
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.5
,8) Which of the following statements regarding vectors is FALSE?
A) Vectors are usually autonomously replicating DNA molecules.
B) A useful vector contains multiple restriction sites for insertion of DNA.
C) Vectors are generally over 100,000 base pairs in size.
D) Cloning vectors frequently contain sequences necessary for expression of inserted sequences.
E) Cloning vectors include a "marker" to facilitate identification of cells containing them.
Answer: C
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.10
9) Synthetic nucleic acids are useful as
A) DNA probes.
B) primers for PCR.
C) antisense RNAs.
D) DNA probes and antisense RNAs.
E) DNA probes, primers, and antisense RNAs.
Answer: E
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.7
10) Mutagens are useful in biotechnology research for
A) producing organisms with altered phenotypes.
B) producing new organisms which have beneficial traits from two or more organisms.
C) selecting genetic mutants resistant to radioactivity.
D) removing undesirable traits from microbes.
E) producing DNA fragments for cloning.
Answer: A
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.4
11) Which of the following is essential in PCR?
A) DNA polymerase
B) antisense RNAs
C) reverse transcriptase
D) DNA primers
E) both DNA primers and DNA polymerase
Answer: E
Bloom's Taxonomy: Comprehension
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
, 12) If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA
polymerase in the PCR procedure, what would be the result?
A) DNA replication would occur more slowly than normal.
B) DNA replication would not occur at all.
C) Many mistakes would occur.
D) DNA replication would occur twice as fast as normal.
E) DNA replication would stop after one cycle.
Answer: E
Bloom's Taxonomy: Analysis
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
13) What is the function of the high temperature step in PCR (polymerase chain reaction)?
A) activation of the DNA polymerase
B) allowing primers to bind complementary sequences
C) facilitate DNA synthesis
D) deactivate DNA polymerase to pause synthesis
E) break hydrogen bonds for strand separation
Answer: E
Bloom's Taxonomy: Comprehension
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
14) A procedure using both reverse transcriptase and real time PCR can be used to
A) determine the number of copies of a DNA sequence in a sample.
B) determine the number of copies of an RNA sequence in a sample.
C) produce DNA fragments for cDNA cloning.
D) both determine the number of copies of an RNA sequence in a sample and produce fragments
for cDNA cloning.
E) produce RNA fragments for cloning.
Answer: D
Bloom's Taxonomy: Application
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
15) In gel electrophoresis, DNA molecules move toward the electrode because they
have an overall charge.
A) negative; negative
B) positive; positive
C) negative; positive
D) positive; negative
E) negative and positive; neutral
Answer: D
Bloom's Taxonomy: Application
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.14
Robert Bauman 5th Edition All Chapters Included With Complete
Solutions 2026/2027
Chapter : Recombinant DNA Technology
8.1 Multiple Choice Questions
1) The use of microorganisms to produce useful products is known as
A) biotechnology.
B) gene modification.
C) genomics.
D) recombinant DNA technology.
E) cloning.
Answer: A
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.1
2) In the 20th century, scientists harnessed the natural metabolic reactions of bacteria to make
for the first time in an industrial setting.
A) soy sauce
B) acetone
C) wine
D) cheese
E) alcohol
Answer: B
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.2
3) Which of the following items is NOT a part of the name of a restriction enzyme?
A) the Gram reaction of the source bacterium
B) the specific epithet of the source bacterium
C) Roman numerals to indicate its order of discovery
D) the strain of the source bacterium
E) the genus of the source bacterium
Answer: A
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.8
,4) The natural role of restriction enzymes in bacteria is to
A) make conjugation more efficient.
B) allow transposons to move to another place in the chromosome.
C) protect the cell from invading phages.
D) allow cells to accept foreign DNA.
E) provide the cell with new phenotypes, such as antibiotic resistance.
Answer: C
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9
5) Which of the following is a product of recombinant DNA technology?
A) rice that produces vitamin C
B) bacteria that produce insulin
C) yeast that produces alcohol
D) bacteria that produce acetone
E) microbes that digest cellulose
Answer: B
Bloom's Taxonomy: Application
Section: Applications of Recombinant DNA Technology
Learning Outcome: 8.20
6) Which of the following restriction enzyme sites would produce blunt-ended fragments? (The
arrow represents the cutting site of the enzyme.)
A) C↓CGG
B) G↓GATCC
C) G↓AATTC
D) CCC↓GGG
E) A↓AGCTT
Answer: D
Bloom's Taxonomy: Application
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.9
7) The enzyme reverse transcriptase
A) synthesizes DNA to repair gaps in a DNA strand.
B) synthesizes DNA from an RNA template.
C) synthesizes RNA from an RNA template.
D) synthesizes RNA from a DNA template.
E) can synthesize DNA or RNA from an RNA template.
Answer: B
Bloom's Taxonomy: Knowledge
Section: The Role of Recombinant DNA Technology in Biotechnology
Learning Outcome: 8.5
,8) Which of the following statements regarding vectors is FALSE?
A) Vectors are usually autonomously replicating DNA molecules.
B) A useful vector contains multiple restriction sites for insertion of DNA.
C) Vectors are generally over 100,000 base pairs in size.
D) Cloning vectors frequently contain sequences necessary for expression of inserted sequences.
E) Cloning vectors include a "marker" to facilitate identification of cells containing them.
Answer: C
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.10
9) Synthetic nucleic acids are useful as
A) DNA probes.
B) primers for PCR.
C) antisense RNAs.
D) DNA probes and antisense RNAs.
E) DNA probes, primers, and antisense RNAs.
Answer: E
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.7
10) Mutagens are useful in biotechnology research for
A) producing organisms with altered phenotypes.
B) producing new organisms which have beneficial traits from two or more organisms.
C) selecting genetic mutants resistant to radioactivity.
D) removing undesirable traits from microbes.
E) producing DNA fragments for cloning.
Answer: A
Bloom's Taxonomy: Comprehension
Section: The Tools of Recombinant DNA Technology
Learning Outcome: 8.4
11) Which of the following is essential in PCR?
A) DNA polymerase
B) antisense RNAs
C) reverse transcriptase
D) DNA primers
E) both DNA primers and DNA polymerase
Answer: E
Bloom's Taxonomy: Comprehension
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
, 12) If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA
polymerase in the PCR procedure, what would be the result?
A) DNA replication would occur more slowly than normal.
B) DNA replication would not occur at all.
C) Many mistakes would occur.
D) DNA replication would occur twice as fast as normal.
E) DNA replication would stop after one cycle.
Answer: E
Bloom's Taxonomy: Analysis
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
13) What is the function of the high temperature step in PCR (polymerase chain reaction)?
A) activation of the DNA polymerase
B) allowing primers to bind complementary sequences
C) facilitate DNA synthesis
D) deactivate DNA polymerase to pause synthesis
E) break hydrogen bonds for strand separation
Answer: E
Bloom's Taxonomy: Comprehension
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
14) A procedure using both reverse transcriptase and real time PCR can be used to
A) determine the number of copies of a DNA sequence in a sample.
B) determine the number of copies of an RNA sequence in a sample.
C) produce DNA fragments for cDNA cloning.
D) both determine the number of copies of an RNA sequence in a sample and produce fragments
for cDNA cloning.
E) produce RNA fragments for cloning.
Answer: D
Bloom's Taxonomy: Application
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.12
15) In gel electrophoresis, DNA molecules move toward the electrode because they
have an overall charge.
A) negative; negative
B) positive; positive
C) negative; positive
D) positive; negative
E) negative and positive; neutral
Answer: D
Bloom's Taxonomy: Application
Section: Techniques of Recombinant DNA Technology
Learning Outcome: 8.14
