ACS BIOCHEMISTRY EXAM PREP 2026
QUESTIONS WITH ANSWERS GRADED A+
◉At pH 7, which amino acid is negatively charged? ANSWER: -
Aspartic acid (Asp) is negatively charged at pH 7 due to its carboxyl
group being deprotonated.
◉The peptide bond is best described as: ANSWER: - Planar with partial
double-bond character, which restricts rotation and gives the peptide its
structure.
◉Competitive inhibition affects which parameter? ANSWER: -
Competitive inhibition affects Km only, increasing it while Vmax
remains unchanged.
◉Noncompetitive inhibition results in: ANSWER: - Noncompetitive
inhibition results in the same Km but a decreased Vmax.
◉Which molecule activates PFK-1? ANSWER: - AMP activates PFK-1,
promoting glycolysis when energy levels are low.
◉The rate-limiting step of glycolysis is catalyzed by: ANSWER: -
Phosphofructokinase-1 (PFK-1) catalyzes the rate-limiting step of
glycolysis.
QUESTIONS WITH ANSWERS GRADED A+
◉At pH 7, which amino acid is negatively charged? ANSWER: -
Aspartic acid (Asp) is negatively charged at pH 7 due to its carboxyl
group being deprotonated.
◉The peptide bond is best described as: ANSWER: - Planar with partial
double-bond character, which restricts rotation and gives the peptide its
structure.
◉Competitive inhibition affects which parameter? ANSWER: -
Competitive inhibition affects Km only, increasing it while Vmax
remains unchanged.
◉Noncompetitive inhibition results in: ANSWER: - Noncompetitive
inhibition results in the same Km but a decreased Vmax.
◉Which molecule activates PFK-1? ANSWER: - AMP activates PFK-1,
promoting glycolysis when energy levels are low.
◉The rate-limiting step of glycolysis is catalyzed by: ANSWER: -
Phosphofructokinase-1 (PFK-1) catalyzes the rate-limiting step of
glycolysis.
