Bio 99 midterm 1 Questions With Complete Solutions
3' end Correct Answers has an OH
5' end Correct Answers phosphate group
a probe in nucleic acid hybridization should be... Correct
Answers antiparallel and complementary to the desired gene
sequence
are hydrogen bonds weak or strong? Correct Answers weak
compared to covalent bonds
are RNA viruses an exception to the central dogma Correct
Answers yes (they can perform reverse transcriptase going from
Rna --> DNA) (they don't need DNA, the RNA gets translated
into proteins)
auxotroph Correct Answers mutant defective in synthesizing a
compound (ex: leucine auxotroph cannot make leucine so must
grow on a leucine plate)
can a plasmid without an origin of replication be transformed?
Correct Answers yes because it can uptake the DNA but it
cannot pass it on
central dogma Correct Answers DNA -> RNA -> Protein
centromere Correct Answers chromosome segregation during
division
, crispr cas 9 Correct Answers bacterial immune system used to
cut out/edit foreign DNA sequences --> we can now use these
for gene editing (ex: to remove HIV dna)
dna annealing Correct Answers the winding/base pairing of two
strands into a double stranded helix
dna denaturation Correct Answers the unwinding of the double
helix (is reversible)
DNA is negative or positive Correct Answers negatively
charged because of the phosphate backbone
DNA polymerase adds in Correct Answers 5 --> 3' because of
the 3' OH
DNA polymerase Correct Answers requires a
- DNA template strand
- primer RNA strand
- and dNTP substrates
do plasmids need telomeres? Correct Answers if they are
circular no
if they are linear then yes (because they become unstable/shrink
after each division)
Each nucleosome contains Correct Answers 8 histones
exons Correct Answers coding portions of the sequence (same
exons can be rearranged to make different proteins)
3' end Correct Answers has an OH
5' end Correct Answers phosphate group
a probe in nucleic acid hybridization should be... Correct
Answers antiparallel and complementary to the desired gene
sequence
are hydrogen bonds weak or strong? Correct Answers weak
compared to covalent bonds
are RNA viruses an exception to the central dogma Correct
Answers yes (they can perform reverse transcriptase going from
Rna --> DNA) (they don't need DNA, the RNA gets translated
into proteins)
auxotroph Correct Answers mutant defective in synthesizing a
compound (ex: leucine auxotroph cannot make leucine so must
grow on a leucine plate)
can a plasmid without an origin of replication be transformed?
Correct Answers yes because it can uptake the DNA but it
cannot pass it on
central dogma Correct Answers DNA -> RNA -> Protein
centromere Correct Answers chromosome segregation during
division
, crispr cas 9 Correct Answers bacterial immune system used to
cut out/edit foreign DNA sequences --> we can now use these
for gene editing (ex: to remove HIV dna)
dna annealing Correct Answers the winding/base pairing of two
strands into a double stranded helix
dna denaturation Correct Answers the unwinding of the double
helix (is reversible)
DNA is negative or positive Correct Answers negatively
charged because of the phosphate backbone
DNA polymerase adds in Correct Answers 5 --> 3' because of
the 3' OH
DNA polymerase Correct Answers requires a
- DNA template strand
- primer RNA strand
- and dNTP substrates
do plasmids need telomeres? Correct Answers if they are
circular no
if they are linear then yes (because they become unstable/shrink
after each division)
Each nucleosome contains Correct Answers 8 histones
exons Correct Answers coding portions of the sequence (same
exons can be rearranged to make different proteins)
