MCB 461 Exam 2 - Clickers and Quizzes || with Error-free
Solutions.
Which does NOT belong to non-classical neurotransmitter?
a. Nitric Oxide
b. Anandamide (AEA)
c. ATP
d. Acetylcholine correct answers d
Acetylcholine is a classic neurotransmitter
What is the function of synaptotagmin?
a. Synaptotagmin is a tSNARE that mediates vesicle fusion
b. Synaptotagmin is a vSNARE that mediates vesicle fusion
c. Synaptotagmin binds SNARE complex and inhibits fusion prior to Ca2+ signal
d. Synaptotagmin is a Ca2+ sensor that triggers rearrangement of SNARE complexes for
immediate fusion correct answers d
Which protein uses ATP hydrolysis to dissociate and recycle SNARE complex proteins?
a. Synaptobrevin
b. Complexin
c. N-ethylmaleimide-sensitive fusion protein (NSF)
d. Syntaxin correct answers c
iClicker: Which is TRUE for electrical synapses?
A. They contain only glutamate receptors
B. They allow only ions to diffuse directly between cells
C. They mediate very fast synaptic transmission with no synaptic delay
D. They are present only in embryonic development. correct answers C
iClicker: What was "Vagusstoff" from Dr. Loewi's experiment and how does it cause the heart
#2 to slow down?
A. Glutamate. Binding of glutamate to glutamate receptors causes Na+ influx into heart muscle
cell, leading to depolarization
B. GABA. Binding of GABA to GABA receptor causes rapid influx of Cl- ions into heart muscle
cell, leading to hyperpolarization
C. Acetylcholine. Binding of acetylcholine to muscarinic acetylcholine receptor causes opening
of K+ channels in the heart muscle membrane, leading to hyperpolarization correct answers C
iClicker: Which statement is TRUE for large dense core vesicles?
A. Could contain dopamine
B. Could contain enkephalin
C. Packaged locally at chemically synapse correct answers B
, iClicker: Which neurotransmitter is released from secretory granules?
a. Glutamate
b. Nitric oxide
c. dopamine
d. Substance-P correct answers d
it is a neuropeptide
iClicker: How do you identify glutamatergic neurons?
a. by immunostaining for glutaminase or VGLUT
b. by immunostaining for glutamic acid decarboxylase (GAD) or VIATT
c. by immunostaining for voltage-gated Ca2+ channels
by immunostaining for SNARE proteins correct answers a
Dopamine D2 receptors (D2R) are autoreceptors that play critical roles in drug addiction in
relevant brain circuits. The D2Rs are expressed pre-synaptically in dopaminergic neurons.
However, the D2Rs are also located post-synaptically in non-dopaminergic neurons. Which
experiment is BEST to address the contribution of presynaptic D2Rs but not postsynaptic D2Rs
on dopamine-mediated cocaine-seeking behavior in mice?
a. pretreat the mice with DOPA-decarboxylase inhibitor and test cocaine seeking behavior
b. pretreat the mice with D2R antagonist and test cocaine seeking behavior
c. make global D2R knock-out mice and test these ice for cocaine seeking behavior
d. make mice deficient of D2R only in dopaminergic neurons and test these mice for cocaine
seeking behavior correct answers d
iClicker: What accounts for synaptic delay observed at a chemical synapse as compared to an
electrical synapse?
a. Calcium influx through voltage-gated calcium channels
b. Exocytosis of synaptic vesicles
c. Diffusion of neurotransmitters at synaptic cleft
d. Production of changes in membrane permeability via interaction of the transmitters with
postsynaptic receptors
e. All of the above correct answers e
Which protein couples action potential to exocytosis?
a. Synaptobrevin
b. Synaptotagmin
c. SNAP-25
d. Syntaxin
e. NSF correct answers b
How does alpha-CaMKII, stay "on" or "active" long after intracellular Ca2+ concentration has
fallen back to low level?
a. persistent interaction between regulatory domain and catalytic domain of alpha-CaMKII
Solutions.
Which does NOT belong to non-classical neurotransmitter?
a. Nitric Oxide
b. Anandamide (AEA)
c. ATP
d. Acetylcholine correct answers d
Acetylcholine is a classic neurotransmitter
What is the function of synaptotagmin?
a. Synaptotagmin is a tSNARE that mediates vesicle fusion
b. Synaptotagmin is a vSNARE that mediates vesicle fusion
c. Synaptotagmin binds SNARE complex and inhibits fusion prior to Ca2+ signal
d. Synaptotagmin is a Ca2+ sensor that triggers rearrangement of SNARE complexes for
immediate fusion correct answers d
Which protein uses ATP hydrolysis to dissociate and recycle SNARE complex proteins?
a. Synaptobrevin
b. Complexin
c. N-ethylmaleimide-sensitive fusion protein (NSF)
d. Syntaxin correct answers c
iClicker: Which is TRUE for electrical synapses?
A. They contain only glutamate receptors
B. They allow only ions to diffuse directly between cells
C. They mediate very fast synaptic transmission with no synaptic delay
D. They are present only in embryonic development. correct answers C
iClicker: What was "Vagusstoff" from Dr. Loewi's experiment and how does it cause the heart
#2 to slow down?
A. Glutamate. Binding of glutamate to glutamate receptors causes Na+ influx into heart muscle
cell, leading to depolarization
B. GABA. Binding of GABA to GABA receptor causes rapid influx of Cl- ions into heart muscle
cell, leading to hyperpolarization
C. Acetylcholine. Binding of acetylcholine to muscarinic acetylcholine receptor causes opening
of K+ channels in the heart muscle membrane, leading to hyperpolarization correct answers C
iClicker: Which statement is TRUE for large dense core vesicles?
A. Could contain dopamine
B. Could contain enkephalin
C. Packaged locally at chemically synapse correct answers B
, iClicker: Which neurotransmitter is released from secretory granules?
a. Glutamate
b. Nitric oxide
c. dopamine
d. Substance-P correct answers d
it is a neuropeptide
iClicker: How do you identify glutamatergic neurons?
a. by immunostaining for glutaminase or VGLUT
b. by immunostaining for glutamic acid decarboxylase (GAD) or VIATT
c. by immunostaining for voltage-gated Ca2+ channels
by immunostaining for SNARE proteins correct answers a
Dopamine D2 receptors (D2R) are autoreceptors that play critical roles in drug addiction in
relevant brain circuits. The D2Rs are expressed pre-synaptically in dopaminergic neurons.
However, the D2Rs are also located post-synaptically in non-dopaminergic neurons. Which
experiment is BEST to address the contribution of presynaptic D2Rs but not postsynaptic D2Rs
on dopamine-mediated cocaine-seeking behavior in mice?
a. pretreat the mice with DOPA-decarboxylase inhibitor and test cocaine seeking behavior
b. pretreat the mice with D2R antagonist and test cocaine seeking behavior
c. make global D2R knock-out mice and test these ice for cocaine seeking behavior
d. make mice deficient of D2R only in dopaminergic neurons and test these mice for cocaine
seeking behavior correct answers d
iClicker: What accounts for synaptic delay observed at a chemical synapse as compared to an
electrical synapse?
a. Calcium influx through voltage-gated calcium channels
b. Exocytosis of synaptic vesicles
c. Diffusion of neurotransmitters at synaptic cleft
d. Production of changes in membrane permeability via interaction of the transmitters with
postsynaptic receptors
e. All of the above correct answers e
Which protein couples action potential to exocytosis?
a. Synaptobrevin
b. Synaptotagmin
c. SNAP-25
d. Syntaxin
e. NSF correct answers b
How does alpha-CaMKII, stay "on" or "active" long after intracellular Ca2+ concentration has
fallen back to low level?
a. persistent interaction between regulatory domain and catalytic domain of alpha-CaMKII
